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Literature summary for 3.4.22.62 extracted from

  • Yasuda, Y.; Saito, M.; Yamamura, T.; Yaguchi, T.; Nishizaki, T.
    Extracellular adenosine induces apoptosis in Caco-2 human colonic cancer cells by activating caspase-9/-3 via A(2a) adenosine receptors (2009), J. Gastroenterol., 44, 56-65.
    View publication on PubMed
Search for more literature for 3.4.22.62

Activating Compound

Activating Compound Comment Organism Structure
adenosine extracellular adenosine induces apoptosis by activating caspase-9 and caspase-3 in association with mitochondrial damage via A2a adenosine receptors, induction dose-dependent (1-20 mM) and time-dependent (24-72 h) Homo sapiens

Application

Application Comment Organism
medicine mechanisms of adenosine-induced apoptosis in human colonic cancer cells Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-caspase-3 + H2O Homo sapiens mechanisms of adenosine-induced apoptosis in human colonic cancer cells caspase-3 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P55211
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Caco-2 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 mechanisms of adenosine-induced apoptosis in human colonic cancer cells, cell viability, DNA laddering, expression analysis of adenosine receptors, overexpression of A2a adenosine receptors, determination of mitochondrial membrane potentials, adenosine disrupts mitochondrial membrane potentials and activates caspase-9 and caspase-3, but not caspase-8 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-LEHD-7-amido-4-trifluoromethylcoumarin + H2O i.e. Ac-Leu-Glu-His-Asp-MCA, fluorescence assay, 50 microg cell lysate incubated with 5 microl of 1 mM substrate Homo sapiens acetyl-LEHD + 7-amino-4-trifluoromethylcoumarin
-
 ?
pro-caspase-3 + H2O mechanisms of adenosine-induced apoptosis in human colonic cancer cells Homo sapiens caspase-3 + ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 activity assay at Homo sapiens