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Literature summary for 3.4.22.62 extracted from

  • McDonnell, M.A.; Abedin, M.J.; Melendez, M.; Platikanova, T.N.; Ecklund, J.R.; Ahmed, K.; Kelekar, A.
    Phosphorylation of murine caspase-9 by the protein kinase casein kinase 2 regulates its cleavage by caspase-8 (2008), J. Biol. Chem., 283, 20149-20158.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli, pcmyc vector, pFLAG-CTC vector to generate FLAG-tagged caspase-9 construct Mus musculus

Protein Variants

Protein Variants Comment Organism
S348A introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase Mus musculus
S348G introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase Mus musculus
S348V introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase Mus musculus
S350A introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pro-caspase-9 + H2O Mus musculus auto-processing, apoptosis induced by tumor necrosis factor (TNF)-alpha promotes dephosphorylation of caspase-9 by casein kinase 2 (CK2), phosphorylation by casein kinase 2 decreases susceptibility of caspase-9 to cleavage by active caspase-8 caspase-9 + ?
-
?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9R0T0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of caspase-9 by casein kinase 2 (CK2) on a serine near the site of caspase-8 cleavage, TNF receptor cross-linking promotes dephosphorylation of caspase-9, phosphorylation of caspase-9 by casein kinase 2 (CK2) decreases its susceptibility to cleavage by active caspase-8 Mus musculus

Purification (Commentary)

Purification (Comment) Organism
gel filtration, SDS-PAGE, recombinant protein Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast caspase-9 null mouse embryo fibroblast Mus musculus
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FL5.12 cell
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Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
determination of caspase-9 auto-processing in a cell-free system, phosphorylation of caspase-9 analyzed by Western blot, phosphorylation of caspase-9 by casein kinase 2 (CK2) on a serine near the site of caspase-8 cleavage, CK2 modification of Ser348 on caspase-9 lowers the susceptibility of caspase-9 to cleavage but does not affect caspase-9 auto-processing, substitution of Ser348 abolishes phosphorylation but not cleavage, phosphatase inhibitors delay apoptosis induced by tumor necrosis factor (TNF)-alpha, inhibition of kinase activity or loss of the kinase accelerates apoptosis and promotes early caspase-9 activation in response to TNF-alpha, modification of pro-caspase-9 in the vicinity of its processing motif protects the protease from inappropriate activation by other caspases Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Leu-Glu-His-Asp-p-nitroanilide + H2O i.e. Ac-LEHD-pNA, caspase-9 activity assay Mus musculus acetyl-Leu-Glu-His-Asp + p-nitroaniline
-
?
pro-caspase-9 + H2O auto-processing, apoptosis induced by tumor necrosis factor (TNF)-alpha promotes dephosphorylation of caspase-9 by casein kinase 2 (CK2), phosphorylation by casein kinase 2 decreases susceptibility of caspase-9 to cleavage by active caspase-8 Mus musculus caspase-9 + ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
caspase activity assay at Mus musculus