Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli, pcmyc vector, pFLAG-CTC vector to generate FLAG-tagged caspase-9 construct | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
S348A | introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase | Mus musculus |
S348G | introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase | Mus musculus |
S348V | introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase | Mus musculus |
S350A | introduction of point mutants into full-length murine caspase-9 (mC-9) cDNA, generated by a two-step PCR method, efficiently cleaved by the active initiator caspase | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-caspase-9 + H2O | Mus musculus | auto-processing, apoptosis induced by tumor necrosis factor (TNF)-alpha promotes dephosphorylation of caspase-9 by casein kinase 2 (CK2), phosphorylation by casein kinase 2 decreases susceptibility of caspase-9 to cleavage by active caspase-8 | caspase-9 + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q9R0T0 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation of caspase-9 by casein kinase 2 (CK2) on a serine near the site of caspase-8 cleavage, TNF receptor cross-linking promotes dephosphorylation of caspase-9, phosphorylation of caspase-9 by casein kinase 2 (CK2) decreases its susceptibility to cleavage by active caspase-8 | Mus musculus |
Purification (Comment) | Organism |
---|---|
gel filtration, SDS-PAGE, recombinant protein | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast | caspase-9 null mouse embryo fibroblast | Mus musculus | - |
FL5.12 cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
determination of caspase-9 auto-processing in a cell-free system, phosphorylation of caspase-9 analyzed by Western blot, phosphorylation of caspase-9 by casein kinase 2 (CK2) on a serine near the site of caspase-8 cleavage, CK2 modification of Ser348 on caspase-9 lowers the susceptibility of caspase-9 to cleavage but does not affect caspase-9 auto-processing, substitution of Ser348 abolishes phosphorylation but not cleavage, phosphatase inhibitors delay apoptosis induced by tumor necrosis factor (TNF)-alpha, inhibition of kinase activity or loss of the kinase accelerates apoptosis and promotes early caspase-9 activation in response to TNF-alpha, modification of pro-caspase-9 in the vicinity of its processing motif protects the protease from inappropriate activation by other caspases | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Leu-Glu-His-Asp-p-nitroanilide + H2O | i.e. Ac-LEHD-pNA, caspase-9 activity assay | Mus musculus | acetyl-Leu-Glu-His-Asp + p-nitroaniline | - |
? | |
pro-caspase-9 + H2O | auto-processing, apoptosis induced by tumor necrosis factor (TNF)-alpha promotes dephosphorylation of caspase-9 by casein kinase 2 (CK2), phosphorylation by casein kinase 2 decreases susceptibility of caspase-9 to cleavage by active caspase-8 | Mus musculus | caspase-9 + ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
caspase activity assay at | Mus musculus |