Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | active caspase-8 colocalizes with internalized TNF-R1 receptosomes, direct binding of caspase-8 to TNF-R1 on internalized receptosomes, molecular components of a potential signalling cascade from TNF-R1 to A-SMase via caspase-8 actually localize in the same subcellular compartment | Homo sapiens | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18000 | - |
1 * 41000-43000 + 1 * 18000, mature caspase-8, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | caspase-8 is involved in activation of acid sphingomyelinase induced by TNF, but caspase-8 does not display firm association neither with caspase-7 nor with A-SMase, overview. Direct binding of caspase-8 to TNF-R1 on internalized receptosomes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | cleavage of pro-caspase-8, 54/52 kDa, to the active form of 18 kDa in TNF receptosomes | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
JURKAT cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | caspase-8 is involved in activation of acid sphingomyelinase induced by TNF, but caspase-8 does not display firm association neither with caspase-7 nor with A-SMase, overview. Direct binding of caspase-8 to TNF-R1 on internalized receptosomes | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 41000-43000 + 1 * 18000, mature caspase-8, SDS-PAGE | Homo sapiens |
monomer | 1 * 52000-54000, procaspase-8, SDS-PAGE | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | no change in A-SMase activity in caspase-8-deficient cells. Retransfection of caspase-8-deficient Jurkat cells with an expression plasmid for caspase-8 restores the ability of the transfected cells to respond to TNF stimulation by increased A-SMase activity. Caspase-8-deficient Jurkat cells are almost completely resistant to TNF/CHX treatment | Homo sapiens |
additional information | active caspase-8 colocalizes with internalized TNF-R1 receptosomes | Homo sapiens |
physiological function | caspase-8 and caspase-7 sequentially mediate proteolytic activation of acid sphingomyelinase in TNF-R1 receptosomes. TNF-induced A-SMase activation strictly depends on functional caspase-8 and caspase-7 expression | Homo sapiens |