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Literature summary for 3.4.22.61 extracted from

  • Srinivasula, S.M.; Ahmad, M.; Fernandes-Almeri, T.; Liwack, G.; Alnemri, E.S.
    Molecular ordering of the Fas-apoptotic pathway: the fas/APO-1 protease Mch5 is a CrmA-inhibitable protease that activates multiple Ced-3/ICE-like cysteien proteases (1996), Proc. Natl. Acad. Sci. USA, 93, 14486-14491.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
proMch5 lacking the two N-terminal FADD-like domains are subcloned in the bacterial expression vector pET21b in frame with an N-terminal T7 tag and a C-terminal His6tag. Bacterial expression generates a mature enzyme composed of two subunits, which are derived from the precursor by processing at Asp227, Asp233, Asp391 and Asp401 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cowpox serpin CrmA potent inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the enzyme is responsible for activating a protease cascade after Fas-receptor ligation, leading to cell death. Mch5 is the most upstream protease that receives the activation signal from the Fas-receptor to initiate the apoptotic protease cascade that leads to activation of ICE-like proteases (TX, ICE, and ICE-relIII), Ced-3-like proteases (CPP32, Mch2, Mch3, Mch4 and Mch6) and the ICH-1 protease ?
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Organism

Organism UniProt Comment Textmining
Homo sapiens
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Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification bacterial expression generates a mature enzyme composed of two subunits, which are derived from the precursor by processing at Asp227, Asp233, Asp391 and Asp401 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is responsible for activating a protease cascade after Fas-receptor ligation, leading to cell death. Mch5 is the most upstream protease that receives the activation signal from the Fas-receptor to initiate the apoptotic protease cascade that leads to activation of ICE-like proteases (TX, ICE, and ICE-relIII), Ced-3-like proteases (CPP32, Mch2, Mch3, Mch4 and Mch6) and the ICH-1 protease Homo sapiens ?
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