Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.22.61 extracted from

  • Watt, W.; Koeplinger, K.A.; Mildner, A.M.; Heinrikson, R.L.; Tomasselli, A.G.; Watenpaugh, K.D.
    The atomic-resolution structure of human caspase-8, a key activator of apoptosis (1999), Structure Fold. Des., 7, 1135-1143.
    View publication on PubMed
Search for more literature for 3.4.22.61

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of the enzyme covalently modified with acetyl-IETD-aldehyde, hanging- and sitting-drop vapor diffusion Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-IETD-aldehyde IC50: 50 nM, covalently modifies the active site C360 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.066
-
 acetyl-IETD-4-nitroanilide pH 7.5, 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
11000
-
 alpha2beta2, 2 * 18000 + 2 * 11000, crystallographic data Homo sapiens
18000
-
 alpha2beta2, 2 * 18000 + 2 * 11000, crystallographic data Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the initiator caspase-8 activates other downstream caspases that are incapable of autocatalytic processing and activation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q14790
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-DEVD-4-nitroanilide + H2O
-
 Homo sapiens acetyl-DEVD + 4-nitroaniline
-
 ?
acetyl-IETD-4-nitroanilide + H2O
-
 Homo sapiens acetyl-IETD + 4-nitroaniline
-
 ?
additional information the catalytic triad in caspase-8 comprises C260, H317 and the backbone carbonyl oxygen atom of R258, which points towards the Nepsilon atom of H317 Homo sapiens ?
-
 ?
additional information the initiator caspase-8 activates other downstream caspases that are incapable of autocatalytic processing and activation Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
tetramer alpha2beta2, 2 * 18000 + 2 * 11000, crystallographic data Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00005
-
 IC50: 50 nM, covalently modifies the active site C360 Homo sapiens acetyl-IETD-aldehyde