Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D23A | the mutation shows increased catalytic activity but is unable to cleave PARP | Homo sapiens |
K38A | the mutant shows increased catalytic activity | Homo sapiens |
K40A | the mutant shows reduced catalytic activity | Homo sapiens |
K41A | the mutant shows reduced catalytic activity | Homo sapiens |
S37A | the mutant shows increased catalytic activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Z-VAD-fluoromethylketone | irreversible inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0298 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K40A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
0.0319 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | wild type enzyme, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
0.0357 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K38A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
0.0366 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K39A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
0.0375 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme S37A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
0.0478 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K41A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
0.0687 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme D23A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme does not cleave ICAD | ? | - |
? | |
p23 + H2O | Homo sapiens | better substrate for caspase-7 than caspase-3. p23 is cleaved at site PEVD*G | ? | - |
? | |
poly(ADP ribose) polymerase 1 + H2O | Homo sapiens | better substrate for caspase-7 than caspase-3, PARP-1 is cleaved at the canonical caspase-3/7 site DEVD*G | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P55210 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not cleave ICAD | Homo sapiens | ? | - |
? | |
additional information | no activity with N-acetyl-PEVD-7-amido-4-fluoromethylcoumarin | Homo sapiens | ? | - |
? | |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin + H2O | - |
Homo sapiens | N-acetyl-DEVD + 7-amino-4-fluoromethylcoumarin | - |
? | |
p23 + H2O | better substrate for caspase-7 than caspase-3. p23 is cleaved at site PEVD*G | Homo sapiens | ? | - |
? | |
poly(ADP ribose) polymerase 1 + H2O | better substrate for caspase-7 than caspase-3, PARP-1 is cleaved at the canonical caspase-3/7 site DEVD*G | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Casp7 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K40A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
3.4 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | wild type enzyme, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
3.9 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K39A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
4.1 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K41A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
4.3 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K38A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
4.4 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme S37A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
9.5 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme D23A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
86 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K41A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
97 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K40A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
110 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K39A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
110 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | wild type enzyme, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
120 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme K38A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
120 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme S37A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens | |
140 | - |
N-acetyl-DEVD-7-amido-4-fluoromethylcoumarin | mutant enzyme D23A, in 10 mM PIPES (pH 7.4), 100 mM NaCl, 10% sucrose (w/v), 0.1% CHAPS, 1 mM EDTA and 10 mM dithiothreitol, at 37°C | Homo sapiens |