Protein Variants | Comment | Organism |
---|---|---|
D628A | site-directed mutagenesis, caspase-7 resistant mutant, retains telomerase activity | Homo sapiens |
E286A | site-directed mutagenesis, inactive mutant | Homo sapiens |
E286A/D628A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-DEVD-fluoromethylketone | a caspase-7 inhibitor | Homo sapiens | |
Z-VAD-fluoromethylketone | a Pan caspase inhibitor | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
JURKAT cell | - |
Homo sapiens | - |
K-562 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
human telomerase + H2O | caspase-7 cleaves human telomerase, hTERT, at residues E286 and D628, generation of three major proteolytic fragments, cleavage site determination using caspase-7 resistant substrate mutants D129A, D637A, and D129A/D637A, overview | Homo sapiens | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |