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Literature summary for 3.4.22.6 extracted from

  • Diaz-Mochon, J.J.; Planonth, S.; Bradley, M.
    From 10,000 to 1: Selective synthesis and enzymatic evaluation of fluorescence resonance energy transfer peptides as specific substrates for chymopapain (2009), Anal. Biochem., 384, 101-105.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme is used for a number of biomedical applications Carica papaya
medicine used for pain relief in treatment of herniated invertebral disc Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
0.023
-
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
0.026
-
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
0.026
-
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
0.0502
-
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 7.4 Carica papaya
0.0502
-
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
0.0506
-
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 pH 6.2 Carica papaya
0.0506
-
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Carica papaya mass spectroscopy studies show unequivocally the specificity of chymopapain toward Ala, Pro, Val and Lys for positions P4 to P1 while not presenting high specificity for residues in position P1 ?
-
?

Organism

Organism UniProt Comment Textmining
Carica papaya
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation
-
Carica papaya
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O FRET 2, fluorescence resonance energy transfer peptide 2 Carica papaya methyl red-Abu-Ala-Pro-Val-Lys + Lys(N5-(5-carboxyfluorescein))-NH2
-
?
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya methyl red-Abu-Ala-Pro-Val-Lys + Lys(N5-(5-carboxyfluorescein))-NH2
-
?
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N5-(5-carboxyfluorescein))-NH2 + H2O pH 6.2 or pH 7.4, 10 min, 37°C Carica papaya methyl red-Abu-Ser-Ala-Pro-Val-Lys + Ala-Lys(N5-(5-carboxyfluorescein))-NH2
-
?
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 + H2O FRET 1, fluorescence resonance energy transfer peptide 1 Carica papaya methyl red-Abu-Ser-Ala-Pro-Val-Lys + Ala-Lys(N6-(5-carboxyfluorescein))-NH2
-
?
additional information mass spectroscopy studies show unequivocally the specificity of chymopapain toward Ala, Pro, Val and Lys for positions P4 to P1 while not presenting high specificity for residues in position P1 Carica papaya ?
-
?
N-alpha-benzoyl-DL-arginine-para-nitroanilide + H2O
-
Carica papaya ?
-
?
N-alpha-benzoyl-L-arginine ethyl ester + H2O
-
Carica papaya ?
-
?
N-alpha-benzoylphenylalanine-arginine-4-methylcoumarin-7-amide + H2O
-
Carica papaya ?
-
?

Synonyms

Synonyms Comment Organism
Chymopapain
-
Carica papaya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Carica papaya
37
-
pH 6.2 or pH 7.4, 10 min Carica papaya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
133
-
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
246
-
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 6.2, in 10 mM sodium acetate, 0.1 mM EDTA and 1 mM cysteine Carica papaya
360
-
methyl red-Abu-Ser-Ala-Pro-Val-Lys-Ala-Lys(N6-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya
590
-
methyl red-Abu-Ala-Pro-Val-Lys-Lys(N5-(5-carboxyfluorescein))-NH2 at pH 7.4, in 100 mM phosphate-buffered saline Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
assay at Carica papaya
7.4
-
assay at Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
additional information
-
chymopapain has a broad working pH range Carica papaya
6.2 7.4 10 min, 37°C Carica papaya