Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for therapeutic intervention in Alzheimer's and Huntington's diseases | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of knockout mice by using a construct for targeting the C57BL/6 caspase-6 locus in ES cells. lamin A/C degradation is markedly reduced in fibroblasts extracted from caspase-6 knock-out mice relative to fibroblasts from wild type mice | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-DEVD-CHO | - |
Homo sapiens | |
Ac-IETD-CHO | - |
Homo sapiens | |
Ac-VEID-CHO | - |
Homo sapiens | |
Q-VD-OPH | a Pan caspase inhibitor | Homo sapiens | |
Z-DEVD-fluoromethylketone | - |
Homo sapiens | |
Z-VAD-fluoromethylketone | - |
Homo sapiens | |
Z-VEID-fluoromethylketone | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lamin A + H2O | Mus musculus | - |
? | - |
? | |
lamin A + H2O | Homo sapiens | - |
? | - |
? | |
lamin A + H2O | Mus musculus C57BL/6 | - |
? | - |
? | |
lamin c + H2O | Mus musculus | - |
? | - |
? | |
lamin c + H2O | Homo sapiens | - |
? | - |
? | |
lamin c + H2O | Mus musculus C57BL/6 | - |
? | - |
? | |
additional information | Mus musculus | development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview | ? | - |
? | |
additional information | Homo sapiens | development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview | ? | - |
? | |
additional information | Mus musculus C57BL/6 | development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Mus musculus C57BL/6 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | active recombinant caspase-6 | Homo sapiens | - |
neuroblastoma cell | - |
Homo sapiens | - |
SK-N-AS cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(Ac-VEID)2-Rh110 + H2O | - |
Homo sapiens | ? | - |
? | |
lamin A + H2O | - |
Mus musculus | ? | - |
? | |
lamin A + H2O | - |
Homo sapiens | ? | - |
? | |
lamin A + H2O | - |
Mus musculus C57BL/6 | ? | - |
? | |
lamin c + H2O | - |
Mus musculus | ? | - |
? | |
lamin c + H2O | - |
Homo sapiens | ? | - |
? | |
lamin c + H2O | - |
Mus musculus C57BL/6 | ? | - |
? | |
additional information | development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview | Mus musculus | ? | - |
? | |
additional information | development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview | Homo sapiens | ? | - |
? | |
additional information | development of an assay method for specifically detecting caspase-6 activity in an intact cell environment, evaluation of a 384-well whole-cell chemiluminescent ELISA assay that monitors the proteolytic degradation of endogenously expressed lamin A/C during the early stages of caspase-dependent apoptosis, overview | Mus musculus C57BL/6 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000162 | - |
pH 7.2, 37°C, recombinant enzyme | Homo sapiens | Ac-VEID-CHO | |
0.0000305 | - |
pH 7.2, 37°C, recombinant enzyme | Homo sapiens | Ac-DEVD-CHO | |
0.0000532 | - |
pH 7.2, 37°C, recombinant enzyme | Homo sapiens | Ac-IETD-CHO | |
0.0001286 | - |
pH 7.2, 37°C, recombinant enzyme | Homo sapiens | Z-VEID-fluoromethylketone | |
0.0004023 | - |
pH 7.2, 37°C, recombinant enzyme | Homo sapiens | Z-DEVD-fluoromethylketone | |
0.002112 | - |
pH 7.2, 37°C, recombinant enzyme | Homo sapiens | Z-VAD-fluoromethylketone |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | staurosporine induces caspase-6 | up |
General Information | Comment | Organism |
---|---|---|
physiological function | caspase-6 is a cysteinyl protease implicated in neurodegenerative conditions including Alzheimer's and Huntington's diseases | Homo sapiens |