Cloned (Comment) | Organism |
---|---|
expression of His-tagged apo-caspase-6 and caspase-6 in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
recombinant caspase-6 comprising residues 24179 and 194293 after self-cleavage of the zymogen, overall structure of the four caspase-6 p202/p102 tetramers in the asymmetric unit, and caspase-6 in complex with inhibitor Ac-VEID-CHO, hanging-drop vapor diffusion method, mixing of 0.001 ml protein solution containing 20 mM sodium acetate, pH 5.5, 50 mM NaCl, and 0.5 mM Tris(hydroxypropyl)phosphine with 0.001 ml of reservoir solution consisting of 3.3 M sodium nitrate, 0.1 M Tris, pH 7.4, 0.5% ethyl acetate, and 5 mM Tris(hydroxypropyl)phosphine, and microseeding, 1 week, 20°C, X-ray diffraction structure determination and analysis at 2.53 A resolution, molecular replacement, structure comparisons, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ac-VEID-CHO | a reversible caspase-6 inhibitor, enzyme-inhibitor complex structure, overview | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P55212 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged apo-caspase-6 and caspase-6 from Escherichia coli by nickel affinity chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Z-VEID-R110 + H2O | caspase-6 recognition peptide covalently linked to two amine groups of the fluorescent dye rhodamine 110, fluorogenic assay | Homo sapiens | 2 Z-VEID + R110 | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | active canonical conformation of the apoenzyme apo-caspase-6, comparison to the apostructure of pH-inactivated caspase-6,crystal structure analysis, overview. Caspase-6 subunits p20 and p10 comprise residues 24179 and 194293, respectively | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
caspase-6 activity is optimal around neutral pH and is almost entirely lost at pH 5.0 and below | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | caspase-6 is a key component in the pathway of neurodegenerative diseases such as Alzheimer's disease and Huntington's diseases | Homo sapiens |