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Literature summary for 3.4.22.59 extracted from

  • Soares, J.; Lowe, M.M.; Jarstfer, M.B.
    The catalytic subunit of human telomerase is a unique caspase-6 and caspase-7 substrate (2011), Biochemistry, 50, 9046-9055.
    View publication on PubMed
Search for more literature for 3.4.22.59

Protein Variants

Protein Variants Comment Organism
D129A site-directed mutagenesis, inactive mutant Homo sapiens
E637A site-directed mutagenesis, inactive mutant Homo sapiens
additional information generation of stable caspase-6 knockdown cell lines using K562 cells. Mutations at residues D442, D444, D516, D768, and D936 do not prevent cleavage in vitro Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Z-VAD-fluoromethylketone a Pan caspase inhibitor Homo sapiens
Z-VEID-fluoromethylketone a caspase-6 inhibitor Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
human telomerase + H2O Homo sapiens caspase-6 cleaves human telomerase, hTERT, during apoptosis in cultured cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
 Homo sapiens
-
JURKAT cell
-
 Homo sapiens
-
K-562 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
human telomerase + H2O caspase-6 cleaves human telomerase, hTERT, during apoptosis in cultured cells Homo sapiens ?
-
 ?
human telomerase + H2O caspase-6 cleaves human telomerase, hTERT, at residues D129 and D637, generation of three major proteolytic fragments, cleavage site determination using caspase-6 resistant substrate mutants D129A, D637A, and D129A/D637A, overview Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 assay at Homo sapiens

General Information

General Information Comment Organism
evolution the caspase-cleavage motifs in teleomerases from old world monkeys and apes, and the caspase-6 sites appear conserved in all primates Homo sapiens
physiological function telomerase can inhibit or activate apoptosis depending upon cell type and experimental conditions, mechanism for telomerase downregulation through caspase-mediated cleavage Homo sapiens