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Literature summary for 3.4.22.59 extracted from

  • Klaiman, G.; Champagne, N.; Leblanc, A.C.
    Self-activation of Caspase-6 in vitro and in vivo: Caspase-6 activation does not induce cell death in HEK293T cells (2009), Biochim. Biophys. Acta, 1793, 592-601.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information caspase-6 can be entirely self-activated through self-processing, the prodomain of Casp6 entirely prevents self-processing and activation in vivo but not in vitro, removal of the pro-domain promotes Casp6 activation Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
Casp6 genetic organization, expression of His-tagged wild-type and mutant pro-caspase-6 in Escherichia coli and in HEK-293T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
D163A site-directed mutagenesis, uncleavable and catalytically inactive mutant of pro-caspase-6 Homo sapiens
D179A site-directed mutagenesis, the mutant of pro-caspase-6 is altered in one cleavage site residue, and is about half as catalytically active as the wild-type enzyme Homo sapiens
D179A/D193A site-directed mutagenesis, the mutant of pro-caspase-6 is altered in two cleavage site residues, and is catalytically inactive Homo sapiens
D193A site-directed mutagenesis, uncleavable and catalytically inactive mutant of pro-caspase-6 Homo sapiens
D23A site-directed mutagenesis, the mutant of pro-caspase-6 is altered in one cleavage site residue, but is still catalytically active similar to the wild-type enzyme Homo sapiens
D23A/D179A site-directed mutagenesis, the mutant of pro-caspase-6 is altered in two cleavage site residues, and is only slightly to not catalytically active Homo sapiens
D23A/D179A/D193A site-directed mutagenesis, the triple mutant of pro-caspase-6 is altered in three cleavage site residues becoming uncleavable for activation and thus is catalytically inactive or very poorly active Homo sapiens
D23A/D193A site-directed mutagenesis, the mutant of pro-caspase-6 is altered in two cleavage site residues, and is catalytically inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information because the mutant pro-Casp6C163A is not cleaved in serum-deprived conditions and is a dominant negative inhibitor of Casp6 activation Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens caspase-6 is a short pro-domain caspase that is activated early in Alzheimer disease, its activation does not induce apoptosis in HEK-293T cells, overview ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification caspase-6 activates itself through self-processing, processing at either intersubunit linker sites D23, D179 and D193, the prodomain of Casp6 entirely prevents self-processing and activation in vivo but not in vitro, removal of the pro-domain promotes Casp6 activation. Processing of caspase-6 mutants by recombinant caspase-3, overview Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293T cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information caspase-6 is a short pro-domain caspase that is activated early in Alzheimer disease, its activation does not induce apoptosis in HEK-293T cells, overview Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
Casp6
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Homo sapiens