Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | caspase-6 can be entirely self-activated through self-processing, the prodomain of Casp6 entirely prevents self-processing and activation in vivo but not in vitro, removal of the pro-domain promotes Casp6 activation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
Casp6 genetic organization, expression of His-tagged wild-type and mutant pro-caspase-6 in Escherichia coli and in HEK-293T cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D163A | site-directed mutagenesis, uncleavable and catalytically inactive mutant of pro-caspase-6 | Homo sapiens |
D179A | site-directed mutagenesis, the mutant of pro-caspase-6 is altered in one cleavage site residue, and is about half as catalytically active as the wild-type enzyme | Homo sapiens |
D179A/D193A | site-directed mutagenesis, the mutant of pro-caspase-6 is altered in two cleavage site residues, and is catalytically inactive | Homo sapiens |
D193A | site-directed mutagenesis, uncleavable and catalytically inactive mutant of pro-caspase-6 | Homo sapiens |
D23A | site-directed mutagenesis, the mutant of pro-caspase-6 is altered in one cleavage site residue, but is still catalytically active similar to the wild-type enzyme | Homo sapiens |
D23A/D179A | site-directed mutagenesis, the mutant of pro-caspase-6 is altered in two cleavage site residues, and is only slightly to not catalytically active | Homo sapiens |
D23A/D179A/D193A | site-directed mutagenesis, the triple mutant of pro-caspase-6 is altered in three cleavage site residues becoming uncleavable for activation and thus is catalytically inactive or very poorly active | Homo sapiens |
D23A/D193A | site-directed mutagenesis, the mutant of pro-caspase-6 is altered in two cleavage site residues, and is catalytically inactive | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | because the mutant pro-Casp6C163A is not cleaved in serum-deprived conditions and is a dominant negative inhibitor of Casp6 activation | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | caspase-6 is a short pro-domain caspase that is activated early in Alzheimer disease, its activation does not induce apoptosis in HEK-293T cells, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | caspase-6 activates itself through self-processing, processing at either intersubunit linker sites D23, D179 and D193, the prodomain of Casp6 entirely prevents self-processing and activation in vivo but not in vitro, removal of the pro-domain promotes Casp6 activation. Processing of caspase-6 mutants by recombinant caspase-3, overview | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEK-293T cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | caspase-6 is a short pro-domain caspase that is activated early in Alzheimer disease, its activation does not induce apoptosis in HEK-293T cells, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Casp6 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |