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Literature summary for 3.4.22.58 extracted from

  • Fassy, F.; Krebs, O.; Rey, H.; Komara, B.; Gillard, C.; Capdevila, C.; Yea, C.; Faucheu, C.; Blanchet, A.M.; Miossec, C.; Diu-Hercend, A.
    Enzymic activity of two caspases related to interleukin-1beta-converting enzyme (1998), Eur. J. Biochem., 253, 76-83.
    View publication on PubMed
Search for more literature for 3.4.22.58

Cloned(Commentary)

Cloned (Comment) Organism
the enzyme is expressed in baculovirus-infected insect cells, as a 30000 Da protein lacking the propeptide Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
acetyl-DEVD-aldehyde
-
 Homo sapiens
acetyl-YVAD-aldehyde
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.86
-
 succinyl-YVAD-7-amido-4-methylcoumarin pH 7.5, 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens the production of the enzyme induces the activation of an endogenous 32000 Da putative cysteine protease (CPP32) like caspase ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification auto maturation Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
interleukin-1beta + H2O 50 nM enzyme partly cleaves interleukin-1beta into the 28000 Da form and a very faint 17500 Da band can be detected, with other dilutions of TY no cleavage is detected Homo sapiens ?
-
 ?
additional information very little efficiency on caspase-1 substrates Homo sapiens ?
-
 ?
additional information the production of the enzyme induces the activation of an endogenous 32000 Da putative cysteine protease (CPP32) like caspase Homo sapiens ?
-
 ?
succinyl-YVAD-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens succinyl-YVAD + 7-amino-4-methylcoumarin
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0008
-
 acety-YVAD-aldehyde pH 7.5, 37°C Homo sapiens
0.0014
-
 acety-DEVD-aldehyde pH 7.5, 37°C Homo sapiens