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Literature summary for 3.4.22.56 extracted from

  • Jin, D.; Ojcius, D.M.; Sun, D.; Dong, H.; Luo, Y.; Mao, Y.; Yan, J.
    Leptospira interrogans induces apoptosis in macrophages via caspase-8- and caspase-3-dependent pathways (2009), Infect. Immun., 77, 799-809.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.56

Activating Compound

Activating Compound Comment Organism Structure
FADD adapter molecule, required for caspase-3 activation Mus musculus
FADD adapter molecule, required for caspase-3 activation Homo sapiens
additional information In some cells, active caspase-8 is sufficient to activate caspase-3 directly. In other cells, however, caspase-8 indirectly mediates caspase-3 activation by cleaving the proapoptotic Bcl-2 family member Bid, which induces mitochondrial cytochrome c release. Leptospira interrogans induces apoptosis in macrophages and hepatocytes via caspase-8- and caspase-3-dependent pathways Mus musculus
additional information In some cells, active caspase-8 is sufficient to activate caspase-3 directly. In other cells, however, caspase-8 indirectly mediates caspase-3 activation by cleaving the proapoptotic Bcl-2 family member Bid, which induces mitochondrial cytochrome c release. Leptospira interrogans induces apoptosis in macrophages and hepatocytes via caspase-8- and caspase-3-dependent pathways Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 activation Homo sapiens
additional information inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 activation Mus musculus
N-carbobenzyloxy-DEVD-fluoromethyl ketone effects on the activation of caspase-3 through cell infection with Leptospira interrogans Homo sapiens
N-carbobenzyloxy-DEVD-fluoromethyl ketone effects on the activation of caspase-3 through cell infection with Leptospira interrogans Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADD–caspase-8-dependent pathway, independently of mitochondrial cytochrome c–caspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview ?
-
 ?
additional information Homo sapiens caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADD–caspase-8-dependent pathway, independently of mitochondrial cytochrome c–caspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview ?
-
 ?
procaspase-6 + H2O Mus musculus activation requires caspase-3 caspase-6 + ?
-
 ?
procaspase-6 + H2O Homo sapiens activation requires caspase-3 caspase-6 + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification caspase-9 and caspase-8 activate procaspase-3 to caspase-3 by cleavage Mus musculus
proteolytic modification caspase-9 and caspase-8 activate procaspase-3 to caspase-3 by cleavage Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
-
 Homo sapiens
-
hepatocyte
-
 Mus musculus
-
hepatocyte
-
 Homo sapiens
-
J-774A.1 cell
-
 Mus musculus
-
macrophage peritoneal Mus musculus
-
macrophage peritoneal Homo sapiens
-
umbilical vein endothelial cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Mus musculus
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin + H2O
-
 Mus musculus acetyl-Asp-Glu-Val-Asp + 7-amino-4-trifluoromethylcoumarin
-
 ?
acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens acetyl-Asp-Glu-Val-Asp + 7-amino-4-trifluoromethylcoumarin
-
 ?
additional information caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADD–caspase-8-dependent pathway, independently of mitochondrial cytochrome c–caspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview Mus musculus ?
-
 ?
additional information caspase-3 is an effector caspase. The intrinsic death pathway involves mitochondrial release of cytochrome c, which interacts with Apaf-1 and dATP to promote procaspase-9 autoactivation, which in turn activates downstream effectors such as caspase-3, -6, and -7. Increased caspase-3 activation is involved in apoptosis, inhibition of caspase-8 impairs Leptospira interrogans-induced caspase-3 and -6 activation, as well as PARP and lamin A/C cleavage and apoptosis, overview. Leptospira interrogans-induced apoptosis in macrophages is mediated by caspase-3 and -6 activation through a FADD–caspase-8-dependent pathway, independently of mitochondrial cytochrome c–caspase-9-dependent signaling. Caspase-3 is required for the activation of caspase-6 and seems to be involved in caspase-9 activation through a feedback amplification loop. Regulation, overview Homo sapiens ?
-
 ?
procaspase-6 + H2O activation Mus musculus caspase-6 + ?
-
 ?
procaspase-6 + H2O activation Homo sapiens caspase-6 + ?
-
 ?
procaspase-6 + H2O activation requires caspase-3 Mus musculus caspase-6 + ?
-
 ?
procaspase-6 + H2O activation requires caspase-3 Homo sapiens caspase-6 + ?
-
 ?

Synonyms

Synonyms Comment Organism
cysteine aspartic acid-specific protease
-
 Mus musculus
cysteine aspartic acid-specific protease
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Mus musculus
7.4
-
 assay at Homo sapiens