Crystallization (Comment) | Organism |
---|---|
in complex with inhibitor Asp-Glu-Val-Asp-chloromethylketone | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D179A | increase both in kcat- and KM-value. D179 is involved in substrate recognition | Homo sapiens |
DELTA176-181 | decrease both in kcat- and KM-value | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Asp-Glu-Val-Asp-chloromethylketone | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 4.75, 37°C | Homo sapiens | |
0.0048 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 4.75, 37°C | Homo sapiens | |
0.0069 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 7.2, 37°C | Homo sapiens | |
0.008 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 4.75, 37°C | Homo sapiens | |
0.0088 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 7.2, 37°C | Homo sapiens | |
0.0097 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.2, 37°C | Homo sapiens | |
0.0122 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 4.75, 37°C | Homo sapiens | |
0.013 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 4.75, 37°C | Homo sapiens | |
0.0138 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.2, 37°C | Homo sapiens | |
0.0163 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 4.75, 37°C | Homo sapiens | |
0.0255 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 7.2, 37°C | Homo sapiens | |
0.0335 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 7.2, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P42574 | recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, inclusion bodies produced in Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | acetyl-L-Asp-L-Glu-L-Val-L-Asp + 7-amino-4-methylcoumarin | - |
? | |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin + H2O | - |
Homo sapiens | benzoyl-L-Asp-L-Glu-L-Val-L-Asp + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.2, 37°C | Homo sapiens | |
0.03 | 0.55 | benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.2, 37°C | Homo sapiens | |
0.06 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 4.75, 37°C | Homo sapiens | |
0.08 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 4.75, 37°C | Homo sapiens | |
0.16 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 4.75, 37°C | Homo sapiens | |
0.33 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 4.75, 37°C | Homo sapiens | |
1.1 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 4.75, 37°C | Homo sapiens | |
1.1 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 7.2, 37°C | Homo sapiens | |
1.37 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 4.75, 37°C | Homo sapiens | |
1.43 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 7.2, 37°C | Homo sapiens | |
1.54 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant DELTA176-181, pH 7.2, 37°C | Homo sapiens | |
3.5 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 7.2, 37°C | Homo sapiens | |
6.01 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.2, 37°C | Homo sapiens | |
7.08 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | wild-type, pH 7.2, 37°C | Homo sapiens | |
19.04 | - |
benzoyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 7.2, 37°C | Homo sapiens | |
19.56 | - |
acetyl-L-Asp-L-Glu-L-Val-L-Asp-7-amido-4-methylcoumarin | mutant D179A, pH 7.2, 37°C | Homo sapiens |