Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae and Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C303A | inactive | Homo sapiens |
D152A | pro-caspase mutant, like the wild-type, this mutant is efficiently processed between the large and the small subunit, however, it is not further processed to seperate the prodomain from the large subunit | Homo sapiens |
D316A | fusion of the linker to the large subunit, toxic when expressed in yeast | Homo sapiens |
D316A/D330A | abolishes auto-processing and reduces enzymatic activity dramatically, 840fold decrease in activity | Homo sapiens |
D330A | fusion of the linker to the small subunit, slightly greater deleterious effect on enzyme activity than fusion to the large subunit (D316A), toxic when expressed in yeast | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | caspase-2 is not subject to inhibition by members of the IAP family of apoptosis inhibitors | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P42575 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein from Escherichia coli | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-DEVD-7-amino-4-trifluoromethyl coumarin + H2O | 37°C, pH 7.0, 10 mM dithiothreitol | Homo sapiens | 7-amino-4-trifluoromethyl coumarin + Ac-DEVD | - |
? | |
BID + H2O | 37°C, pH 7.0, 10 mM dithiothreitol | Homo sapiens | ? | - |
? | |
catalytically inactive pro-caspase-2 + H2O | 37°C, pH 7.0, 10 mM dithiothreitol | Homo sapiens | caspase-2 | - |
? | |
additional information | PARP is not cleaved | Homo sapiens | ? | - |
? | |
pro-caspase-7 + H2O | 37°C, pH 7.0, 10 mM dithiothreitol | Homo sapiens | caspase-7 | - |
? | |
Z-VDVAD-7-amino-4-trifluoromethyl coumarin + H2O | 37°C, pH 7.0, 10 mM dithiothreitol | Homo sapiens | 7-amino-4-trifluoromethyl coumarin + Z-VDVAD | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | acive form, SDS-PAGE | Homo sapiens |
monomer | inactive form, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
caspase-2 | - |
Homo sapiens |