Protein Variants | Comment | Organism |
---|---|---|
C129S | protease-inactive mutant form | Rattus norvegicus |
N358D | activity-attenuated mutant, in contrast to wild type, which autolyses quickly in the absence of Ca2+, the mutant N358D expressed in Sf-9 cells can be partially purified, N358D is very unstable in solutions with high salt concentrations, autolysis of N358D is NaCl-dependent | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | calpain 3/p94 is activated by Ca2+ and undergoes very rapid autolytic degradation even in the absence of Ca2+ | Rattus norvegicus | |
Na+ | calpain 3/p94 is activated by Na+ and undergoes Na+-dependent, but not Cs+-dependent, autolysis in the absence of Ca2+. Na+ and Ca2+ complementarily activate autolysis of calpain3/p94 at physiological concentrations. Na+ and Ca2+ direct calpain3/p94 to proteolyze different substrates | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-actinin-3 + H2O | Rattus norvegicus | - |
? | - |
? | |
alpha-fodrin + H2O | Rattus norvegicus | - |
? | - |
? | |
tropomyosin + H2O | Rattus norvegicus | - |
? | - |
? | |
troponin I + H2O | Rattus norvegicus | - |
? | - |
? | |
troponins T + H2O | Rattus norvegicus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-actinin-3 + H2O | - |
Rattus norvegicus | ? | - |
? | |
alpha-fodrin + H2O | - |
Rattus norvegicus | ? | - |
? | |
tropomyosin + H2O | - |
Rattus norvegicus | ? | - |
? | |
troponin I + H2O | - |
Rattus norvegicus | ? | - |
? | |
troponins T + H2O | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
calpain 3 | - |
Rattus norvegicus |
p94 | - |
Rattus norvegicus |