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Literature summary for 3.4.22.51 extracted from

  • Doyle, P.S.; Zhou, Y.M.; Hsieh, I.; Greenbaum, D.C.; McKerrow, J.H.; Engel, J.C.
    The Trypanosoma cruzi protease cruzain mediates immune evasion (2011), PLoS Pathog., 7, e1002139.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
K11777 the chemotherapeutical drug targets the major cysteine protease cruzain and disrupts amastigote intracellular development. The mechanism of drug resistance, in primary epimastigotes, is due to secretion of inactive, unprocessed cruzain Trypanosoma cruzi

Localization

Localization Comment Organism GeneOntology No. Textmining
cell surface the signaling factor NF-kB P65 colocalizes with cruzain on the cell surface of intracellular wild-type parasites Trypanosoma cruzi 9986
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
human NF-kappaB P65 + H2O Trypanosoma cruzi
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?
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?

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
human NF-kappaB P65 + H2O
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Trypanosoma cruzi ?
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?

General Information

General Information Comment Organism
malfunction cruzain-deficient Trypanosoma cruzi rapidly activates host macrophages via NF-kB P65 and is unable to survive intracellularly within macrophages. No significant IL-12 expression occurs in macrophages infected with wild-type Trypanosoma cruzi and treated with lipopolysacchrides and brefeldin A, confirming impairment of macrophage activation pathways Trypanosoma cruzi
physiological function infection with wild-type parasites appears to induce cruzain-mediated proteolysis of NF-kappaB P65 leading to unresponsiveness of the host macrophage during early 60 min of infection. This immune evasion mechanism may be critical for Trypaosoma cruzi survival during early natural infection with a low number of trypmastigotes. The signaling factor NF-kappaB P65 colocalizes with cruzain on the cell surface of intracellular wild-type parasites, and is proteolytically cleaved. Transcription factor NF-kappaB P65 is translocated to the nucleus and activated only in macrophages infected with cruzain-deficient parasites Trypanosoma cruzi