BRENDA - Enzyme Database show
show all sequences of 3.4.22.51

Synthesis of macrocyclic trypanosomal cysteine protease inhibitors

Chen, Y.T.; Lira, R.; Hansell, E.; McKerrow, J.H.; Roush, W.R.; Bioorg. Med. Chem. Lett. 18, 5860-5863 (2008)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
-
Trypanosoma brucei rhodesiense
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
-
Trypanosoma cruzi
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
-
Trypanosoma brucei rhodesiense
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
-
Trypanosoma cruzi
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-(morpholin-4-ylcarbonyl)-L-phenylalaninamide
-
Trypanosoma cruzi
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
-
Trypanosoma brucei rhodesiense
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
-
Trypanosoma cruzi
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Trypanosoma brucei rhodesiense
-
-
-
Trypanosoma cruzi
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Phe-Arg-AMC
-
696645
Trypanosoma cruzi
?
-
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
696645
Trypanosoma brucei rhodesiense
?
-
-
-
?
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000004
-
-
Trypanosoma cruzi
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
0.0001
-
-
Trypanosoma brucei rhodesiense
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
0.0002
-
-
Trypanosoma cruzi
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
0.006
-
-
Trypanosoma brucei rhodesiense
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
0.01
-
-
Trypanosoma brucei rhodesiense
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
0.01
-
above 0.01 mM
Trypanosoma cruzi
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.000004
-
-
Trypanosoma cruzi
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
0.0001
-
-
Trypanosoma brucei rhodesiense
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
0.0002
-
-
Trypanosoma cruzi
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
0.006
-
-
Trypanosoma brucei rhodesiense
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
0.01
-
-
Trypanosoma brucei rhodesiense
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
0.01
-
above 0.01 mM
Trypanosoma cruzi
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
-
Trypanosoma brucei rhodesiense
(3S,5R,10R)-N-benzyl-3-(2-methylpropyl)-2-oxo-10-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazecane-5-carboxamide
-
Trypanosoma cruzi
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
-
Trypanosoma brucei rhodesiense
(3S,5R,11R)-N-benzyl-3-(2-methylpropyl)-2-oxo-11-[(E)-2-(phenylsulfonyl)ethenyl]-1,4-diazacycloundecane-5-carboxamide
-
Trypanosoma cruzi
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-(morpholin-4-ylcarbonyl)-L-phenylalaninamide
-
Trypanosoma cruzi
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
-
Trypanosoma brucei rhodesiense
Nalpha-[(2R,3E)-2-benzyl-4-(phenylsulfonyl)but-3-enoyl]-N-[(4-methylpiperazin-1-yl)carbonyl]-L-phenylalaninamide
-
Trypanosoma cruzi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Phe-Arg-AMC
-
696645
Trypanosoma cruzi
?
-
-
-
?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
696645
Trypanosoma brucei rhodesiense
?
-
-
-
?
Other publictions for EC 3.4.22.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732453
Merlino
-
Amidines bearing benzofuroxan ...
Trypanosoma cruzi
MedChemComm
3
90-101
2012
-
-
-
-
-
-
22
-
-
-
-
1
-
1
-
-
-
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-
-
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2
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22
-
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-
1
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732673
Uehara
Cruzipain promotes Trypanosoma ...
Trypanosoma cruzi, Trypanosoma cruzi Dm28c
PLoS Negl. Trop. Dis.
6
e1958
2012
-
-
-
-
-
-
7
-
1
-
-
-
-
6
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
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7
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
717475
Pizzo
Synthesis of 2-hydrazolyl-4-th ...
Trypanosoma cruzi
Chem. Biol. Drug Des.
77
166-172
2011
-
-
-
-
-
-
19
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
19
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
718182
Acosta
A striking common O-linked N-a ...
Trypanosoma cruzi
Parasite Immunol.
33
363-370
2011
-
-
-
-
-
-
-
-
3
-
-
-
-
4
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
718307
Doyle
The Trypanosoma cruzi protease ...
Trypanosoma cruzi
PLoS Pathog.
7
e1002139
2011
-
-
-
-
-
-
1
-
1
-
-
1
-
2
-
-
-
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-
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1
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1
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1
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1
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-
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-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
717894
Borchhardt
-
Biochemical evaluation of a se ...
Trypanosoma cruzi
J. Braz. Chem. Soc.
21
142-150
2010
-
1
1
-
-
-
41
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
40
-
1
1
-
-
-
-
40
41
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
717917
Trossini
Cruzain inhibition by hydroxym ...
Trypanosoma cruzi
J. Enzyme Inhib. Med. Chem.
25
62-67
2010
-
-
-
-
-
-
4
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
718066
Trossini
-
Molecular modeling suggests cr ...
Trypanosoma cruzi
Lett. Drug Des. Discov.
7
528-533
2010
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
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-
-
-
-
-
-
5
-
-
-
-
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-
-
-
-
-
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-
-
-
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-
-
1
-
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-
-
4
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
696760
Romeiro
Synthesis, trypanocidal activi ...
Trypanosoma cruzi, Trypanosoma cruzi Tulahuen 2
Bioorg. Med. Chem.
17
641-652
2009
-
-
-
1
-
-
17
-
1
-
-
-
-
6
-
-
-
-
-
-
-
-
-
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-
-
-
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-
-
-
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-
3
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-
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1
-
-
3
17
-
-
1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700567
Pereira
Cysteine peptidases in Herpeto ...
Trypanosoma cruzi, Trypanosoma cruzi Dm28c
Parasitology
136
45-54
2009
-
-
-
-
-
-
2
-
-
-
-
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-
8
-
-
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-
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2
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710673
Huson
Modulation of the immunogenici ...
Trypanosoma congolense
Vet. Res.
40
52
2009
-
-
-
-
-
-
1
-
-
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-
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4
-
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1
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1
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1
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1
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1
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-
-
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-
-
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-
-
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710780
Ortiz
Genes of cathepsin L-like prot ...
Trypanosoma rangeli
Acta Trop.
112
249-259
2009
-
1
-
-
-
-
1
-
-
-
1
7
-
7
-
-
-
-
-
-
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14
-
-
-
-
-
-
-
-
-
-
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-
7
-
-
-
-
-
-
7
-
-
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7
7
-
-
-
-
-
-
-
-
14
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713023
Cortez
Cathepsin L-like genes of Tryp ...
Trypanosoma vivax
Mol. Cell. Probes
23
44-51
2009
-
-
-
-
-
-
-
-
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2
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-
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-
-
677418
Stempin
Cruzipain and SP600125 induce ...
Trypanosoma cruzi
Acta Trop.
106
119-127
2008
-
1
-
-
-
-
-
-
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-
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3
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2
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1
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2
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696523
Scharfstein
Angiotensin-converting enzyme ...
Trypanosoma cruzi
Biol. Chem.
389
1015-1024
2008
1
-
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2
-
2
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4
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1
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1
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2
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2
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1
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696645
Chen
Synthesis of macrocyclic trypa ...
Trypanosoma brucei rhodesiense, Trypanosoma cruzi
Bioorg. Med. Chem. Lett.
18
5860-5863
2008
-
-
-
-
-
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7
-
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3
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-
-
-
-
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-
2
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-
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6
-
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6
7
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-
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-
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2
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696710
Zanatta
Convergent synthesis and cruza ...
Trypanosoma cruzi
Bioorg. Med. Chem.
16
10236-10243
2008
-
-
1
-
-
-
28
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
9
-
-
1
-
-
-
-
9
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
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-
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-
-
-
-
-
-
696729
Porcal
New trypanocidal hybrid compou ...
Trypanosoma cruzi, Trypanosoma cruzi Tulahuen 2
Bioorg. Med. Chem.
16
6995-7004
2008
-
-
-
-
-
-
10
-
-
-
-
-
-
8
-
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1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
9
-
-
-
-
-
-
-
9
10
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
-
-
1
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-
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-
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697814
Elias
Cysteine peptidases in the tom ...
Trypanosoma cruzi, Trypanosoma cruzi Dm28c
Exp. Parasitol.
120
343-352
2008
-
-
-
-
-
-
-
-
-
-
1
-
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9
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1
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-
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-
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-
-
-
-
697939
dos Reis
The role of conserved residues ...
Trypanosoma cruzi
FEBS Lett.
582
485-490
2008
-
-
-
-
-
-
16
-
-
-
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-
-
3
-
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1
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1
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1
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16
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16
16
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-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
698164
SantAnna
All Trypanosoma cruzi developm ...
Trypanosoma cruzi, Trypanosoma cruzi Y
Histochem. Cell Biol.
130
1187-1198
2008
-
-
-
-
-
-
1
-
2
-
-
-
-
12
-
-
1
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2
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1
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2
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1
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2
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-
-
-
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-
-
-
-
-
-
-
-
698293
Acosta
Sulfates are main targets of i ...
Trypanosoma cruzi, Trypanosoma cruzi Tulahuen 2
Int. Immunol.
20
461-470
2008
-
-
-
-
-
-
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-
1
-
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-
7
-
1
1
-
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1
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-
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1
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1
1
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1
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
698473
Brak
Identification of a new class ...
Trypanosoma cruzi
J. Am. Chem. Soc.
130
6404-6410
2008
-
-
-
-
-
-
7
-
-
-
-
-
-
3
-
-
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21
-
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-
-
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-
-
-
-
-
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-
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7
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-
-
-
-
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21
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
699388
Fricker
Metal compounds for the treatm ...
Trypanosoma cruzi
J. Inorg. Biochem.
102
1839-1845
2008
-
-
-
-
-
-
6
1
-
-
-
-
-
5
-
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1
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-
-
-
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1
-
1
-
-
-
1
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6
-
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-
-
-
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6
6
-
1
-
-
-
-
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1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
700564
Fampa
Protease expression analysis i ...
Trypanosoma cruzi
Parasitology
135
1093-1100
2008
-
-
-
-
-
-
2
-
-
-
1
-
-
2
-
-
-
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1
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2
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
700875
Abdulla
RNA interference of Trypanosom ...
Trypanosoma brucei
PLoS Negl. Trop. Dis.
2
e298
2008
-
1
-
-
1
-
-
-
-
-
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2
-
6
-
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2
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1
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1
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2
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-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
701365
Cazorla
Prime-boost immunization with ...
Trypanosoma cruzi, Trypanosoma cruzi RA
Vaccine
26
1999-2009
2008
-
-
1
-
-
-
-
-
-
-
1
-
-
5
-
-
1
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-
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1
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1
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-
1
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1
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1
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1
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-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
679761
Reis
The propeptide of cruzipain--a ...
Trypanosoma cruzi
FEBS J.
274
1224-1234
2007
-
-
1
-
-
-
1
-
-
-
1
-
-
5
-
-
1
-
-
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-
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1
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-
1
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5
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1
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1
5
-
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-
1
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1
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
679892
dos Reis
The substrate specificity of c ...
Trypanosoma cruzi
FEMS Microbiol. Lett.
259
215-220
2006
-
-
-
-
-
-
-
21
-
-
-
-
-
3
-
-
-
-
-
-
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21
-
1
-
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21
1
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-
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-
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21
-
-
-
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-
-
21
-
1
-
-
21
1
-
-
-
-
-
-
-
-
-
667653
Polticelli
Probing the cruzain S2 recogni ...
Trypanosoma cruzi
Biochemistry
44
2781-2789
2005
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
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1
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3
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3
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3
-
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1
-
-
3
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
667951
Fujii
Discovery of potent thiosemica ...
Trypanosoma cruzi
Bioorg. Med. Chem. Lett.
15
121-123
2005
-
-
-
-
-
-
10
-
-
-
-
-
-
4
-
-
-
-
-
-
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-
-
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10
-
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-
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10
10
-
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-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668551
Barboza
Structural analysis of the N-g ...
Trypanosoma cruzi
FEBS J.
272
3803-3815
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
-
-
-
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-
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-
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-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667144
Aoki
Cruzipain, a major Trypanosoma ...
Trypanosoma cruzi
Am. J. Physiol.
286
C206-C212
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
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1
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1
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-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668458
Judice
Carboxydipeptidase activities ...
Trypanosoma cruzi
Eur. J. Biochem.
271
1046-1053
2004
-
-
-
-
-
-
-
7
-
-
-
-
-
7
-
-
-
-
-
-
-
-
7
-
-
-
-
7
2
2
-
-
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-
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-
-
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-
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7
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
7
2
2
-
-
-
-
-
-
-
-
638890
Sterin-Borda
Cruzipain induces autoantibodi ...
Trypanosoma cruzi
Eur. J. Immunol.
33
2459-2468
2003
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
2
-
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1
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-
-
-
-
-
-
-
-
1
-
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1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
667936
Chiyanzu
Synthesis and evaluation of is ...
Trypanosoma cruzi
Bioorg. Med. Chem. Lett.
13
3527-3530
2003
-
-
-
-
-
-
16
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
-
-
16
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
668522
Duschak
Trypanosoma cruzi: partial cha ...
Trypanosoma cruzi
Exp. Parasitol.
104
122-130
2003
-
-
-
-
-
-
1
-
-
-
1
-
-
3
-
-
1
-
-
-
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
10
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670178
Barboza
Presence of sialic acid in N-l ...
Trypanosoma cruzi
Mol. Biochem. Parasitol.
127
69-72
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
670179
Berasain
Specific cleavage sites on hum ...
Trypanosoma cruzi
Mol. Biochem. Parasitol.
130
23-29
2003
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638875
Alvarez
Expression in insect cells of ...
Trypanosoma cruzi
Protein Expr. Purif.
26
467-475
2002
-
-
1
1
-
-
-
-
-
-
2
-
-
2
-
1
1
-
-
-
1
-
3
-
-
-
-
1
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
1
1
-
-
1
-
3
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
638884
Lalmanach
Congopain from Trypanosoma con ...
Trypanosoma congolense
Biol. Chem.
383
739-749
2002
3
-
-
-
-
-
3
-
-
-
1
-
-
5
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
1
1
-
1
-
-
3
-
-
-
-
-
-
-
3
1
-
-
-
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
638885
Lima
Heparan sulfate modulates kini ...
Trypanosoma cruzi
J. Biol. Chem.
277
5875-5881
2002
-
-
-
-
-
-
2
2
-
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
638887
Ramos
Trypanosoma cruzi: cruzipain a ...
Trypanosoma cruzi
Exp. Parasitol.
100
121-130
2002
-
-
-
-
-
-
2
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
638889
Schnapp
Cruzipain induces both mucosal ...
Trypanosoma cruzi
Infect. Immun.
70
5065-5074
2002
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638876
Alves
Analysis of the S2 subsite spe ...
Trypanosoma cruzi
Mol. Biochem. Parasitol.
117
137-143
2001
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
-
-
-
-
-
-
5
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
5
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
638883
Judice
Comparison of the specificity, ...
Trypanosoma cruzi
Eur. J. Biochem.
268
6578-6586
2001
-
-
-
-
-
-
-
4
-
1
-
-
-
2
-
1
-
-
-
-
-
-
5
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
1
-
-
-
-
-
5
-
1
-
-
4
1
-
-
-
-
-
-
-
-
-
638888
Salvati
Modulation of the catalytic ac ...
Trypanosoma cruzi
Eur. J. Biochem.
268
3253-3258
2001
-
-
-
-
-
-
2
4
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
2
4
4
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638891
Stoka
The high stability of cruzipai ...
Trypanosoma cruzi
FEBS Lett.
469
29-32
2000
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
638879
Cazzulo
Cruzipain, the major cysteine ...
Trypanosoma cruzi
Biol. Chem.
378
1-10
1997
1
-
1
1
-
-
13
2
1
-
-
5
-
3
-
1
-
-
-
-
-
-
19
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
-
1
-
-
-
13
-
2
1
-
-
5
-
-
1
-
-
-
-
-
19
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
638880
Del Nery
-
Characterization of the substr ...
Trypanosoma cruzi
Biochem. J.
323
427-433
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
1
-
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-
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-
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-
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-
-
-
-
-
2
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
638882
Gillmor
Structural determinants of spe ...
Trypanosoma cruzi
Protein Sci.
6
1603-1611
1997
-
-
-
1
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
638877
Cazzulo
Hydrolysis of synthetic peptid ...
Trypanosoma cruzi
Cell. Mol. Biol.
42
691-696
1996
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
638886
McGrath
The crystal structure of cruza ...
Trypanosoma cruzi
J. Mol. Biol.
247
251-259
1995
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638881
Eakin
Production of crystallizable c ...
Trypanosoma cruzi
J. Biol. Chem.
268
6115-6118
1993
-
-
1
1
2
-
-
2
-
-
1
-
-
4
-
-
1
-
-
1
-
-
3
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
2
-
-
1
-
-
-
-
1
-
1
-
-
3
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
638892
Cazzulo
On the post-translational modi ...
Trypanosoma cruzi
FEMS Microbiol. Lett.
79
411-416
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
638878
Cazzulo
Some kinetic properties of a c ...
Trypanosoma cruzi
Biochim. Biophys. Acta
1037
186-191
1990
3
-
-
-
-
1
4
1
-
-
2
-
-
1
-
-
-
-
-
1
2
-
6
-
-
-
1
1
3
-
1
-
-
-
-
3
-
-
-
-
-
1
-
4
-
1
-
-
2
-
-
-
-
-
-
1
2
-
6
-
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1
1
3
-
1
-
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-
-