Literature summary for 3.4.22.51 extracted from

Reis, F.C.; Costa, T.F.; Sulea, T.; Mezzetti, A.; Scharfstein, J.; Broemme, D.; Menard, R.; Lima, A.P.
The propeptide of cruzipain--a potent selective inhibitor of the trypanosomal enzymes cruzipain and brucipain, and of the human enzyme cathepsin F (2007), FEBS J., 274, 1224-1234.

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Cloned(Commentary)

Cloned (Comment)	Organism
full-length propeptide of cruzipain is cloned by PCR and expressed in Escherichia coli as a fusion protein with a 6xHis tag at the N-terminus	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
propeptide of cruzipain	PCZ, is also a good inhibitor of brucipain from Trypanosoma brucei. The propeptide of cruzipain does not inhibit human cathepsins S, K or B or papain at the tested concentrations, and moderately inhibits human cathepsin V. Human cathepsin F is very efficiently inhibited (Ki of 32 picomol/L)	Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
14020	-	MALDI-TOF	Trypanosoma cruzi

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using an Ni-nitrilotriacetic acid-agarose resin	Trypanosoma cruzi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O	-	Trypanosoma cruzi	Z-Phe-Arg + 7-amino-4-methylcoumarin	-	?

Synonyms

Synonyms	Comment	Organism
cruzipain	-	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	complete inhibition by the propeptide of cruzipain	Trypanosoma cruzi

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.018	-	propeptide of cruzipain	at pH 6.5 the propeptide of cruzipain inactivates cruzipain competitively	Trypanosoma cruzi
0.027	-	propeptide of cruzipain	at pH 6.0	Trypanosoma cruzi
0.034	-	propeptide of cruzipain	at pH 7.0	Trypanosoma cruzi
0.0565	-	propeptide of cruzipain	at pH 5.5	Trypanosoma cruzi
0.2637	-	propeptide of cruzipain	at pH5.0	Trypanosoma cruzi

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Release 2023.1 (January 2023)