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Literature summary for 3.4.22.51 extracted from

  • Eakin, A.E.; McGrath, M.E.; McKerrow, J.H.; Fletterick, R.J.; Craik, C.S.
    Production of crystallizable cruzain, the major cysteine protease from Trypanosoma cruzi (1993), J. Biol. Chem., 268, 6115-6118.
    View publication on PubMed
Search for more literature for 3.4.22.51

Cloned(Commentary)

Cloned (Comment) Organism
enzyme and truncated version Trypanosoma cruzi

Crystallization (Commentary)

Crystallization (Comment) Organism
recombinant protein and truncated version Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
C25A enzyme is inactive Trypanosoma cruzi
E219P variant with altered cleavage recognition site Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin truncated enzyme Trypanosoma cruzi
0.00096
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin
-
 Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23540
-
 electrospray mass spectrometry Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi Q598P5
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzoyl-FR-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?
benzoyl-YR-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Trypanosoma cruzi ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 E219P mutant, stable for 10 h Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.16
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin truncated enzyme Trypanosoma cruzi
4.49
-
 benzyloxycarbonyl-Phe-Arg-aminomethyl coumarin
-
 Trypanosoma cruzi