Cloned (Comment) | Organism |
---|---|
expression in Sf9 insect cells | Autographa californica nucleopolyhedrovirus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
antipain | 0.00005 mM, complete inhibition | Autographa californica nucleopolyhedrovirus | |
chicken cystatin | 0.0001 mM, complete inhibition | Autographa californica nucleopolyhedrovirus | |
E64 | 0.0001 mM, complete inhibition | Autographa californica nucleopolyhedrovirus | |
iodoacetate | 0.05 mM, 80% inhibition | Autographa californica nucleopolyhedrovirus | |
leupeptin | 0.00005 mM, complete inhibition | Autographa californica nucleopolyhedrovirus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Z-Arg-Arg-7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.022 | - |
Z-Leu-Arg 7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.039 | - |
Z-Phe-Arg 7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.043 | - |
Z-Val-Arg-7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.058 | - |
Z-Val-Val-Arg 7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Autographa californica nucleopolyhedrovirus | P25783 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Autographa californica nucleopolyhedrovirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Autographa californica nucleopolyhedrovirus | N-benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O | - |
Autographa californica nucleopolyhedrovirus | N-benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O | - |
Autographa californica nucleopolyhedrovirus | N-benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Autographa californica nucleopolyhedrovirus | N-benzyloxycarbonyl-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
N-benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O | - |
Autographa californica nucleopolyhedrovirus | N-benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin | - |
? | |
Z-Leu-Arg 7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | Z-Leu-Arg + 7-amino-4-methylcoumarin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
Z-Val-Val-Arg 7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.73 | - |
Z-Arg-Arg-7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.73 | - |
Z-Leu-Arg 7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.94 | - |
Z-Val-Arg-7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus | |
0.95 | - |
Z-Phe-Arg 7-amido-4-methylcoumarin | - |
Autographa californica nucleopolyhedrovirus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | bell-shaped profile, hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin and Z-Arg-Arg-7-amino-4-methylcoumarin | Autographa californica nucleopolyhedrovirus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7.5 | hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin | Autographa californica nucleopolyhedrovirus |
4 | 7.5 | hydrolysis of Z-Arg-Arg-7-amino-4-methylcoumarin | Autographa californica nucleopolyhedrovirus |