Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | separase can be activated by incubating immunoglobulin G-Sepharose 6 containing ZZ TEV4-separase with anaphase-initiated Xenopus cytostatic factor to degrade its inhibitor securin at room temperature for 1 h | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in HEK-293T cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
securin | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.293 | - |
acetyl-Asp-Arg-Glu-Ile-Nle-Arg-7-amido-4-methylcoumarin | at 37°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Rad21 + H2O | Homo sapiens | - |
fragments of Rad21 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HEL cell | - |
Homo sapiens | - |
HL-60 cell | - |
Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, 1 week, no significant loss of activity | Homo sapiens |
23°C, 48 h, 17% loss of activity | Homo sapiens |
4°C, 48 h, 12% loss of activity | Homo sapiens |
temperature, storage medium, duration, loss of activity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-Asp-Arg-Glu-Ile-Nle-Arg-7-amido-4-methylcoumarin + H2O | acetyl-Asp-Arg-Glu-Ile-Nle-Arg is the Rad21-peptidyl | Homo sapiens | acetyl-Asp-Arg-Glu-Ile-Nle-Arg + 7-amino-4-methylcoumarin | - |
? | |
additional information | does not cleave Leu-Glu-His-Asp-CHO-7-amido-4-methylcoumarin | Homo sapiens | ? | - |
? | |
Rad21 + H2O | - |
Homo sapiens | fragments of Rad21 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 55 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
no appreciable activity is observed at 4°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
- |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | - |
Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | separase is overexpressed and mislocalized in a wide range of human cancers, including breast, prostate, and osteosarcoma | up |
General Information | Comment | Organism |
---|---|---|
physiological function | separase plays a pivotal role in the separation of sister chromatids at anaphase by cleaving its substrate cohesin Rad21 | Homo sapiens |