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Literature summary for 3.4.22.48 extracted from

  • Mootz, J.M.; Malone, C.L.; Shaw, L.N.; Horswill, A.R.
    Staphopains modulate Staphylococcus aureus biofilm integrity (2013), Infect. Immun., 81, 3227-3238.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.48

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain ER2566 Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
C237A inactive ScpA mutant Staphylococcus aureus
C243A inactive SspB mutant Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
E-64
-
 Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Staphylococcus aureus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21000
-
 x * 21000, ScpA, SDS-PAGE Staphylococcus aureus
22000
-
 x * 22000, SspB, SDS-PAGE Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus aureus AH1263
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Select nickel affinity column chromatography Staphylococcus aureus

Renatured (Commentary)

Renatured (Comment) Organism
using 50 mM sodium phosphate, 150 mM sodium chloride, 1 mM EDTA, 0.5 M arginine (pH 6.0), 30% (v/v) glycerol, 1 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-carboxyfluorescein-Lys-Lys-Ala-Ala-Glu-Ala-Ser-Lys-(QXL520)-OH + H2O substrate for ScpA Staphylococcus aureus ?
-
 ?
5-carboxyfluorescein-Lys-Lys-Ala-Ala-Glu-Ala-Ser-Lys-(QXL520)-OH + H2O substrate for ScpA Staphylococcus aureus AH1263 ?
-
 ?
Bz-Pro-Phe-Arg-4-nitroanilide + H2O substrate for SspB Staphylococcus aureus ?
-
 ?
Bz-Pro-Phe-Arg-4-nitroanilide + H2O substrate for SspB Staphylococcus aureus AH1263 ?
-
 ?
elastin agar + H2O
-
 Staphylococcus aureus ?
-
 ?
elastin agar + H2O
-
 Staphylococcus aureus AH1263 ?
-
 ?
milk agar + H2O
-
 Staphylococcus aureus ?
-
 ?
milk agar + H2O
-
 Staphylococcus aureus AH1263 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 21000, ScpA, SDS-PAGE Staphylococcus aureus
? x * 22000, SspB, SDS-PAGE Staphylococcus aureus

Synonyms

Synonyms Comment Organism
SCPA
-
 Staphylococcus aureus
SspB
-
 Staphylococcus aureus

Expression

Organism Comment Expression
Staphylococcus aureus staphopain levels are decreased under biofilm-forming conditions up

General Information

General Information Comment Organism
physiological function staphopains SspB and ScpA modulate Staphylococcus aureus biofilm integrity. ScpA enzyme has a conserved ability to inhibit biofilm formation Staphylococcus aureus