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Literature summary for 3.4.22.48 extracted from
- Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli (2005), Biochem. J., 385, 181-187.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| co-expression of His-tagged staphopain A and its inhibitor staphostatin A in Escherichia coli strain BL21(DE3) in inclusion bodies, recombinant enzyme is toxic for Escherichia coli cells, therefore a co-expression with the enzyme inhibitor is required for recombinant enzyme production, method optimization | Staphylococcus aureus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of an inactive mutant, active site mutation of staphopain A results in abrogation of production of both co-expressed proteins | Staphylococcus aureus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by refolding and nickel affinity chromatography, method optimization | Staphylococcus aureus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| recombinant co-expressed His-tagged staphopain A and inhibitor staphostatin A from Escherichia coli strain BL21(DE3) inclusion bodies by treatment with 6 M guanidinium hydrochloride, and dilution in buffer, at pH 6.0, with 40% glycerol content or with arginine and Tween 20 supplementation, method optimization | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| staphopain A | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Staphylococcus aureus |
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