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Literature summary for 3.4.22.48 extracted from

  • Dubin, G.
    Defense against own arms: staphylococcal cysteine proteases and their inhibitors (2003), Acta Biochim. Pol., 50, 715-724.
    View publication on PubMed
Search for more literature for 3.4.22.48

Cloned(Commentary)

Cloned (Comment) Organism
ssp and scp operons, genomic organization of ssp and scp genes, overview Staphylococcus epidermidis
ssp and scp operons, genomic organization of ssp and scp genes, overview Staphylococcus warneri
ssp and scp operons, genomic organization of ssp and scp genes, overview, overexpression of scp operon genes in Escherichia coli Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure determination of staphopain A in complex with inhibitor E64, folding pattern Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
additional information inactivation of gene sspA by transposon insertion exertes a polar effect on genes sspB and sspC expression, the inactivation of all three genes results in attenuation of virulence Staphylococcus aureus

Inhibitors

Inhibitors Comment Organism Structure
E64 ScpA-inhibitor binding structure Staphylococcus aureus
additional information staphostin homologue genes are probably encoding enzyme inhibitors Staphylococcus epidermidis
additional information staphostin homologue genes are probably encoding enzyme inhibitors Staphylococcus warneri
staphostatin A i.e. ScpB, intracellular, endogenous specific inhibitor of ScpA forming noncovalent complexes, structure determination, slight cleaving of the inhibitor by the enzyme Staphylococcus aureus
staphostatin B i.e. SspC, intracellular, endogenous specific inhibitor of SspB forming noncovalent complexes, structure determination, slight cleaving of the inhibitor by the enzyme Staphylococcus aureus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted as zymogen Staphylococcus aureus
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
kininogen + H2O Staphylococcus aureus activation of human protein by SspA, kinin generation is responsible for infection associated pain and endema kinin + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-
Staphylococcus epidermidis
-
-
-
Staphylococcus warneri
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the enzyme staphopain B is synthesized as preproenzyme, the prefragment is cleaved resulting in the proenzyme, after secretion the proenzyme is proteolytically activated to the mature enzyme Staphylococcus aureus

Reaction

Reaction Comment Organism Reaction ID
broad endopeptidase action on proteins including elastin, but rather limited hydrolysis of small-molecule substrates. Assays are conveniently made with hemoglobin, casein or Z-Phe-Arg-NHMec as substrate active site structure and topology of ScpA Staphylococcus aureus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha1-proteinase inhibitor + H2O human protein, inactivation by SspA Staphylococcus aureus ?
-
 ?
Gelatin + H2O staphopain B Staphylococcus aureus ?
-
 ?
kininogen + H2O activation of human protein by SspA, kinin generation is responsible for infection associated pain and endema Staphylococcus aureus kinin + ?
-
 ?
kininogen + H2O human protein, activation by SspA Staphylococcus aureus kinin + ?
-
 ?
additional information staphopains A and B are cysteine proteases, staphopain shows no activity with casein Staphylococcus aureus ?
-
 ?

Synonyms

Synonyms Comment Organism
SCPA
-
 Staphylococcus aureus
SspB
-
 Staphylococcus aureus
staphopain A
-
 Staphylococcus aureus
staphopain B
-
 Staphylococcus aureus