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Literature summary for 3.4.22.48 extracted from

  • Dubin, G.; Chmiel, D.; Mak, P.; Rakwalska, M.; Rzychon, M.; Dubin, A.
    Molecular cloning and biochemical characterisation of proteases from Staphylococcus epidermidis (2001), Biol. Chem., 382, 1575-1582.
    View publication on PubMed
Search for more literature for 3.4.22.48

Cloned(Commentary)

Cloned (Comment) Organism
DNA and partial amino acid sequence determination and analysis Staphylococcus epidermidis

Inhibitors

Inhibitors Comment Organism Structure
alpha2-Macroglobulin from human Staphylococcus epidermidis
E-64 strong Staphylococcus epidermidis
Hg2+ strong Staphylococcus epidermidis
iodoacetamide
-
 Staphylococcus epidermidis
additional information no inhibition by O-phenanthroline, diisofluorophosphate, phenylmethanesulfonyl fluoride, human kininogens and cystatins A,C, and D Staphylococcus epidermidis
Ni2+
-
 Staphylococcus epidermidis
p-hydroxymercuribenzoate
-
 Staphylococcus epidermidis

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Staphylococcus epidermidis
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19832
-
 x * 19832, DNA sequence determination Staphylococcus epidermidis

Organism

Organism UniProt Comment Textmining
Staphylococcus epidermidis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from culture supernatant, apparant homogenity Staphylococcus epidermidis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 purified enzyme, spectrophotometrical assay Staphylococcus epidermidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-1-antitrypsin + H2O limited proteolysis Staphylococcus epidermidis alpha-1-antitrypsin proteolytically cleaved into peptide fragments
-
 ?
HMW-kininogen + H2O limited proteolysis Staphylococcus epidermidis HMW-kininogen proteolytically cleaved into peptide fragments
-
 ?
protein + H2O
-
 Staphylococcus epidermidis peptide fragments
-
 ?

Subunits

Subunits Comment Organism
? x * 19832, DNA sequence determination Staphylococcus epidermidis

Synonyms

Synonyms Comment Organism
Ecp
-
 Staphylococcus epidermidis
extracellular cysteine protease
-
 Staphylococcus epidermidis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Staphylococcus epidermidis