Cloned (Comment) | Organism |
---|---|
the gene fragment encoding the K2 domain (Ala1157-Gly1334) of Kgp from Porphyromonas gingivalis W83 is cloned into a modified vector pET-32a. The constructed plasmid is transformed into Escherichia coli BL21(DE3) competent cells for protein expression | Porphyromonas gingivalis |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of a domain within the haemagglutinin region of Kgp is reported here. The K2 domain structure is a jellyroll fold with two anti-parallel beta-sheets. This fold topology is shared with adhesive domains from functionally diverse receptors such as MAM domains, ephrin receptor ligand binding domains and a number of carbohydrate binding modules | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using Ni-NTA chromatography and gel filtration | Porphyromonas gingivalis |
Synonyms | Comment | Organism |
---|---|---|
KGP | - |
Porphyromonas gingivalis |
Lys-gingipain | - |
Porphyromonas gingivalis |
General Information | Comment | Organism |
---|---|---|
physiological function | the K2 domain of KgP induces haemolysis of erythrocytes in a dose-dependent manner that is augmented by the blocking of anion transport | Porphyromonas gingivalis |