Any feedback?
Literature summary for 3.4.22.45 extracted from
- The helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Hua Enhancer 1 methyltransferase activity in vitro (2011), J. Gen. Virol., 92, 2222-2226.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | zucchini yellow mosaic virus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | generation of different N- and C-terminal truncated DELTA HC-Pro constructs, i.e. comprising residues 93-456, 114-456, and 139-456, or 1-322, 1-350, and 1-372, construction of recombinant mutant MBP:HA-HC-ProFRNK, MBP:HAHC-ProFINK, and truncated MBP:HA-HC-ProFRNK. HC-ProFRNK/FINK clearly inhibits Arabidopsis thaliana HEN1 activity in vitro. HC-ProFRNK/FINK, but not the truncated proteins HC-Pro 93-456, and HC-Pro 1-372 displaying decreased in vitro affinity for AtHEN1 binding, inhibits the methyltransferase activity of AtHEN1 in vitro | zucchini yellow mosaic virus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 99000 | - |
x * 99000, recombinant MBP-tagged wild-type enzyme, SDS-PAGE | zucchini yellow mosaic virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | zucchini yellow mosaic virus | the helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Arabidopsis thaliana Hua Enhancer 1 methyltransferase, HEN1, activity in vitro | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| zucchini yellow mosaic virus | - |
ZYMV | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant MBP-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity purification using amylose magnetic beads | zucchini yellow mosaic virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the helper component-proteinase of the Zucchini yellow mosaic virus inhibits the Arabidopsis thaliana Hua Enhancer 1 methyltransferase, HEN1, activity in vitro | zucchini yellow mosaic virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 99000, recombinant MBP-tagged wild-type enzyme, SDS-PAGE | zucchini yellow mosaic virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HC-Pro | - |
zucchini yellow mosaic virus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | zucchini yellow mosaic virus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | zucchini yellow mosaic virus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | with respect to its silencing suppressor function, small RNA binding appears to be the major activity of HC-Pro. HC-Pro also exhibits other suppressor activities. HC-Pro inhibits the Arabidopsis thaliana Hua Enhancer 1, HEN1, activity, for suppression of plant RNA silencing as a mechanism of the pathogen for survival in the host cell, overview. The RNA methyltransferase HEN1 is responsible for the 3'-terminal 2'-O-methylation of sRNAs | zucchini yellow mosaic virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
