Cloned (Comment) | Organism |
---|---|
functional expression of wild-type MBP:HA-HC-Pro:GFP and truncated mutant MBP:HA-HC-Pro:GFP N1, i.e. enzyme tagged with HA, GFP, and maltose-binding protein, in Escherichia coli leads to autoproteolytic cleavage of the green fluorescent protein, GFP | zucchini yellow mosaic virus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the first 93 residues of the N-terminus of ZYMV HC-Pro. Mutation of the conserved Gly456 residue does not affect the autoproteolytic activity of ZYMV HC-Pro | zucchini yellow mosaic virus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
126000 | - |
recombinant MBP:HA-HC-Pro:GFP | zucchini yellow mosaic virus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
zucchini yellow mosaic virus | - |
ZYMV | - |
Synonyms | Comment | Organism |
---|---|---|
HC-Pro | - |
zucchini yellow mosaic virus |
helper component proteinase | - |
zucchini yellow mosaic virus |
General Information | Comment | Organism |
---|---|---|
evolution | amino acids essential for the proteolytic activity of ZYMV HC-Pro are distinct from those of the tobacco etch virus HC-Pro, although the amino acid sequences in the proteolytic active domain are conserved among potyviruses | zucchini yellow mosaic virus |
physiological function | besides its RNA silencing suppressor, RSS, activity, HC-Pro also exhibits protease, the multifunctional helper component proteinase of potyviruses contains an autoproteolytic function that, together with the protein 1 and NIa proteinase, EC 3.4.22.44, processes the polyprotein into mature proteins | zucchini yellow mosaic virus |