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Literature summary for 3.4.22.45 extracted from

  • Torres-Barcelo, C.; Martin, S.; Daros, J.A.; Elena, S.F.
    From hypo- to hypersuppression: effect of amino acid substitutions on the RNA-silencing suppressor activity of the Tobacco etch potyvirus HC-Pro (2008), Genetics, 180, 1039-1049.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.45

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pTEV7DA with an infectious TEV clone used as source of wild-type virus and template for site-directed mutagenesis. Wild-type and mutants are cloned into pBIN61 vector, electroporation of Agrobacterium tumefaciens for transient expression assays and quantification of suppression activity. tobacco etch virus

Protein Variants

Protein Variants Comment Organism
A387E no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
C390W no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
D421N no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
E273A no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
E299A/D300A RNA silencing suppressor activity reduced compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
E360A/D361A RNA silencing suppressor activity reduced compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows mild etching tobacco etch virus
E443K RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
E452D RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
I11L RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
I442M no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
K309N RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
K454T RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
N193Y RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
N194D RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
N200S RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
P311L RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
Q265H RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
R127G no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
R165G RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
R240A/K241A/H242A no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
R247A/K248A no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
V135A no RNA silencing suppressor activity, Nicotiana benthamiana plant inoculated with infectious transcript shows no etching tobacco etch virus
V192A RNA silencing suppressor activity reduced compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows mild etching tobacco etch virus
V355L RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
V419A RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
Y234H RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus
Y344S RNA silencing suppressor activity similar to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows mild etching tobacco etch virus
Y423H RNA silencing suppressor activity increased compared to wild-type, Nicotiana benthamiana plant inoculated with infectious transcript shows etching tobacco etch virus

Organism

Organism UniProt Comment Textmining
tobacco etch virus
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-
-

Synonyms

Synonyms Comment Organism
HC-Pro
-
 tobacco etch virus