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Literature summary for 3.4.22.45 extracted from

  • Jin, Y.; Ma, D.; Dong, J.; Jin, J.; Li, D.; Deng, C.; Wang, T.
    HC-Pro protein of Potato virus Y can interact with three Arabidopsis 20S proteasome subunits in planta (2007), J. Virol., 81, 12881-12888.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
full-length coding sequence of HC-Pro of potato virus Y fused to the GAL4 DNA-binding domain, cloned into pGBKT7 via BamHI/PstI digestion to form pGBKT7-HC-Pro, different plasmid constructs generated, overview of domains and deletion mutants of HC-Pro given, transformation into Saccharomyces cerevisiae after subcloning into pGBKT7 potato virus Y

Protein Variants

Protein Variants Comment Organism
additional information three deletion mutants designed for HC-Pro protein of potato virus Y, HC-Pro1 (residues 98 to 456), HC-Pro2 (residues 1 to 298) and HC-Pro3 (residues 1 to 97) used for yeast two-hybrid analysis potato virus Y

Organism

Organism UniProt Comment Textmining
potato virus Y P18247 genome polyprotein, includes helper component proteinase HC-pro; N strain
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potato virus Y N P18247 genome polyprotein, includes helper component proteinase HC-pro; N strain
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Purification (Commentary)

Purification (Comment) Organism
recombinant HC-Pro protein of potato virus Y and its deletion mutants potato virus Y

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
interaction studies of the HC-Pro protein of potato virus Y with the 20S proteasome complex from Arabidosis thaliana, interaction in living cells confirmed, N-terminal region (residues 1 to 97), central region (residues 98 to 298) and C-terminal region (residues 299 to 456) assigned, N-terminal region of HC-Pro found to be involved in binding to the 20S proteasome complex, virus transmission process and in the interaction with host proteins potato virus Y

Synonyms

Synonyms Comment Organism
HC-Pro protein
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potato virus Y