Cloned (Comment) | Organism |
---|---|
full-length coding sequence of HC-Pro of potato virus Y fused to the GAL4 DNA-binding domain, cloned into pGBKT7 via BamHI/PstI digestion to form pGBKT7-HC-Pro, different plasmid constructs generated, overview of domains and deletion mutants of HC-Pro given, transformation into Saccharomyces cerevisiae after subcloning into pGBKT7 | potato virus Y |
Protein Variants | Comment | Organism |
---|---|---|
additional information | three deletion mutants designed for HC-Pro protein of potato virus Y, HC-Pro1 (residues 98 to 456), HC-Pro2 (residues 1 to 298) and HC-Pro3 (residues 1 to 97) used for yeast two-hybrid analysis | potato virus Y |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
potato virus Y | P18247 | genome polyprotein, includes helper component proteinase HC-pro; N strain | - |
potato virus Y N | P18247 | genome polyprotein, includes helper component proteinase HC-pro; N strain | - |
Purification (Comment) | Organism |
---|---|
recombinant HC-Pro protein of potato virus Y and its deletion mutants | potato virus Y |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
interaction studies of the HC-Pro protein of potato virus Y with the 20S proteasome complex from Arabidosis thaliana, interaction in living cells confirmed, N-terminal region (residues 1 to 97), central region (residues 98 to 298) and C-terminal region (residues 299 to 456) assigned, N-terminal region of HC-Pro found to be involved in binding to the 20S proteasome complex, virus transmission process and in the interaction with host proteins | potato virus Y |
Synonyms | Comment | Organism |
---|---|---|
HC-Pro protein | - |
potato virus Y |