Any feedback?
Literature summary for 3.4.22.45 extracted from
- Potyvirus helper component proteinase (2004), Handbook of Proteolytic Enzymes (Barrett, A. J. ; Rawlings, N. D. ; Woessner, J. F. ; eds. ), 2, 1262-1264.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in vitro translation of transcripts in rabbit reticulocyte lysate or wheat germ extract, expression in Escherichia coli as His-tagged protein | tobacco etch virus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity | tobacco etch virus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | tobacco etch virus | the enzyme is essential for viral infection of plants and acts as post-transcriptional gene silencer by interferring with miRNA-controlled developmental pathways | ? | - |
? | |
| potyvirus helper component + H2O | tobacco etch virus | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| tobacco etch virus | - |
i.e. TEV, ssRNA positive-strand virus, no DNA stage | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant dimeric His-tagged HC-Pro proteinase from Escherichia coli | tobacco etch virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme is essential for viral infection of plants and acts as post-transcriptional gene silencer by interferring with miRNA-controlled developmental pathways | tobacco etch virus | ? | - |
? | |
| additional information | the cleavage site is highly conserved at P4, P2, P1, and P1' positions | tobacco etch virus | ? | - |
? | |
| potyvirus helper component + H2O | - |
tobacco etch virus | ? | - |
? | |
| potyvirus helper component + H2O | the enzyme cleaves at the C-terminal Gly-/-Gly bond | tobacco etch virus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | the enzyme contains three domains with distinct functions | tobacco etch virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HC-Pro | - |
tobacco etch virus |
| More | the enzyme belongs to the C6 peptidase family | tobacco etch virus |
| potyvirus helper component proteinase | - |
tobacco etch virus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
