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Literature summary for 3.4.22.43 extracted from

  • Novinec, M.; Pavsic, M.; Lenarcic, B.
    A simple and efficient protocol for the production of recombinant cathepsin V and other cysteine cathepsins in soluble form in Escherichia coli (2012), Protein Expr. Purif., 82, 1-5.
    View publication on PubMed
Search for more literature for 3.4.22.43

Activating Compound

Activating Compound Comment Organism Structure
additional information the recombinant proenzyme is autocatalytically activated in vitro by incubation at pH 4.0 and 30°C Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant procathepsin V is expressed in soluble form in the cytoplasm of Escherichia coli Rosetta-gami B (DE3) pLysS cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
E-64 irreversible Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens
0.0044
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 x * 37000, recombinant procathepsin V, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
plasminogen + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O60911
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
benzyloxycarbonyl-Leu-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Leu-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?
plasminogen + H2O
-
 Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 37000, recombinant procathepsin V, SDS-PAGE Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
19
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens
156
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7900
-
 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens
35000
-
 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin in 0.1 M sodium acetate buffer pH 5.5 containing 2.5 mM dithiothreitol and 1 mM EDTA, at 25°C Homo sapiens