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Literature summary for 3.4.22.40 extracted from

  • Sebti, S.M.; Celeon, J.C.; Lazo, J.S.
    Purification, characterization, and amino acid composition of rabbit pulmonary bleomycin hydrolase (1987), Biochemistry, 26, 4213-4219.
    View publication on PubMed
Search for more literature for 3.4.22.40

General Stability

General Stability Organism
stabilized by 2 mM dithiothreitol Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
 Oryctolagus cuniculus
Co2+
-
 Oryctolagus cuniculus
Cu2+
-
 Oryctolagus cuniculus
leupeptin
-
 Oryctolagus cuniculus
N-ethylmaleimide
-
 Oryctolagus cuniculus
puromycin
-
 Oryctolagus cuniculus
Zn2+
-
 Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3
-
 bleomycin A2
-
 Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 5 * 50000, SDS-PAGE Oryctolagus cuniculus
250000
-
 gel filtration, PAGE Oryctolagus cuniculus

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
lung
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bleomycin + H2O bleomycin A2 Oryctolagus cuniculus deamido-bleomycin
-
 ?
bleomycin A2 + H2O
-
 Oryctolagus cuniculus ?
-
 ?

Subunits

Subunits Comment Organism
pentamer 5 * 50000, SDS-PAGE Oryctolagus cuniculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 loss of 50% activity after 10 h Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Oryctolagus cuniculus

pH Range

pH Minimum pH Maximum Comment Organism
6 8
-
 Oryctolagus cuniculus