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Literature summary for 3.4.22.38 extracted from

  • Roy, S.; Dattagupta, J.; Biswas, S.
    Expression of recombinant human cathepsin K is enhanced by codon optimization (2012), Process Biochem., 47, 1944-1947.
No PubMed abstract available
Search for more literature for 3.4.22.38

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol
-
 Homo sapiens
L-cysteine
-
 Homo sapiens
additional information zymogen activation occurs at pH 4.0 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells as inclusion body Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
E-64 irreversible inhibitor Homo sapiens
leupeptin
-
 Homo sapiens
methyl methanethiolsulfonate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 Z-Phe-Arg-7-amido-4-methylcoumarin at pH 5.5 and 25°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29000
-
 x * 29000, mature enzyme, SDS-PAGE Homo sapiens
37600
-
 x * 37600, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
calf skin collagen type I + H2O Homo sapiens
-
 ?
-
 ?
Gelatin + H2O Homo sapiens
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P43235
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography and Sephacryl S-100 gel filtration Homo sapiens

Renatured (Commentary)

Renatured (Comment) Organism
using 50 mM Tris-HCl pH 8.0, 500 mM NaCl, 0.5 M urea, 0.7 M arginine, 5 mM EDTA, 1% CHAPS, 15% (v/v) glycerol, 10 mM GSH and 1 mM GSSG Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
calf skin collagen type I + H2O
-
 Homo sapiens ?
-
 ?
Gelatin + H2O
-
 Homo sapiens ?
-
 ?
Z-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens Z-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 29000, mature enzyme, SDS-PAGE Homo sapiens
? x * 37600, recombinant His-tagged enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
CTSK
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0117
-
 at pH 5.5 and 25°C Homo sapiens E-64

General Information

General Information Comment Organism
physiological function catepsin K is the key enzyme in bone homeostasis Homo sapiens