Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with NSC13345, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, pH 5.5, 30% (w/v) PEG-8000 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C25S | inactive | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
E-64 | irreversible inhibitor | Homo sapiens | |
NSC13345 | i.e. 2-[(2-carbamoylsulfanylacetyl)-amino]benzoic acid, good selectivity for cathepsin K over related enzymes | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
soluble calf-skin collagen + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P43235 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Abz-HPGGPQ-EDDnp + H2O | - |
Homo sapiens | ? | - |
? | |
azocasein + H2O | - |
Homo sapiens | ? | - |
? | |
soluble calf-skin collagen + H2O | - |
Homo sapiens | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NSC13345 | at pH 5.5 and 25°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.08 | - |
at pH 5.5 and 25°C | Homo sapiens | NSC13345 |