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Literature summary for 3.4.22.38 extracted from

  • Novinec, M.; Korenc, M.; Caflisch, A.; Ranganathan, R.; Lenarcic, B.; Baici, A.
    A novel allosteric mechanism in the cysteine peptidase cathepsin K discovered by computational methods (2014), Nat. Commun., 5, 3287.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NSC13345, sitting drop vapor diffusion method, using 0.2 M ammonium sulfate, pH 5.5, 30% (w/v) PEG-8000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
C25S inactive Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
E-64 irreversible inhibitor Homo sapiens
NSC13345 i.e. 2-[(2-carbamoylsulfanylacetyl)-amino]benzoic acid, good selectivity for cathepsin K over related enzymes Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
soluble calf-skin collagen + H2O Homo sapiens
-
?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P43235
-
-

Purification (Commentary)

Purification (Comment) Organism
Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-HPGGPQ-EDDnp + H2O
-
Homo sapiens ?
-
?
azocasein + H2O
-
Homo sapiens ?
-
?
soluble calf-skin collagen + H2O
-
Homo sapiens ?
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.028
-
NSC13345 at pH 5.5 and 25°C Homo sapiens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.08
-
at pH 5.5 and 25°C Homo sapiens NSC13345