Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | procathepsin K is activated by incubation at 37°C in the presence of DTT and dextran sulfate at final concentrations of 1 mM and 100 mg/ml, respectively | Rattus norvegicus | |
additional information | procathepsin K is activated by incubation at 37°C in the presence of DTT and dextran sulfate at final concentrations of 1 mM and 100 mg/ml, respectively | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
cloning from a bone cDNA library, expression in Tn cells | Homo sapiens |
recombinant expression in Tn cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | site-directed mutagenesis of the rat residues Ser and Val to the correspondent Ala and Leu, respectively, of the human sequence causes a loss of the P2 Hyp specificity, overview | Homo sapiens |
S134A | site-directed mutagenesis of the rat residue Ser to the correspondent Ala of the human sequence, the mutant looses the P2 Hyp specificity | Rattus norvegicus |
S134A/V160L | site-directed mutagenesis of the rat residues Ser and Val to the correspondent Ala and Leu, respectively, of the human sequence, the mutant looses the P2 Hyp specificity | Rattus norvegicus |
V160L | site-directed mutagenesis of the rat residue Val to the correspondent Leu of the human sequence, the mutant looses the P2 Hyp specificity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant mutant S134A/V160L | Rattus norvegicus | |
0.0075 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant enzyme | Homo sapiens | |
0.016 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant mutant S134A | Rattus norvegicus | |
0.026 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant mutant V160L | Rattus norvegicus | |
0.293 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant wild-type enzyme | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
collagen I + H2O | Rattus norvegicus | - |
? | - |
? | |
collagen I + H2O | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P43235 | - |
- |
Rattus norvegicus | O35186 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-aminobenzoyl-Gly-Pro-Hyp-Gly-Phe(NO2)-Gly-OH + H2O | - |
Rattus norvegicus | ? | - |
? | |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide + H2O | - |
Rattus norvegicus | carbobenzyloxy-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | carbobenzyloxy-Leu-Arg + 7-amino-4-methylcoumarin | - |
? | |
collagen I + H2O | - |
Rattus norvegicus | ? | - |
? | |
collagen I + H2O | - |
Homo sapiens | ? | - |
? | |
collagen I + H2O | rat tail type I collagen | Rattus norvegicus | ? | - |
? | |
collagen I + H2O | rat tail type I collagen | Homo sapiens | ? | - |
? | |
additional information | human cathepsin K preferentially hydrolyses the substrates with Pro at the P2 position, substrate specificity compared to the rat enzyme, overview | Homo sapiens | ? | - |
? | |
additional information | rat cathepsin K preferentially hydrolyses the substrates at the P2 Hyp position, substrate specificity compared to the human enzyme, overview | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the human sequence contains Asp and Tyr at S3 positions 61 and 67, and residues Met, Ala, Leu, Ala and Leu at S2 positions 68, 134, 160, 163, and 209, respectively, comparison with the rat enzyme | Homo sapiens |
More | the human sequence contains Tyr at S3 positions 61 and 67, and residues Met, Ser, Val, Ala, and Leu at S2 positions 68, 134, 160, 163, and 209, respectively, comparison with the human enzyme | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 37 | assay at, substrate dependently | Rattus norvegicus |
25 | 37 | assay at, substrate dependently | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.1 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant mutant S134A/V160L | Rattus norvegicus | |
46.3 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant mutant V160L | Rattus norvegicus | |
56.1 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant enzyme | Homo sapiens | |
95.7 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant wild-type enzyme | Rattus norvegicus | |
97.5 | - |
carbobenzyloxy-Leu-Arg-4-methylcoumaryl-7-amide | pH 7.0, 37°C, recombinant mutant S134A | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |
7 | - |
assay at | Homo sapiens |