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Literature summary for 3.4.22.37 extracted from

  • Uehara, A.; Naito, M.; Imamura, T.; Potempa, J.; Travis, J.; Nakayama, K.; Takada, H.
    Dual regulation of interleukin-8 production in human oral epithelial cells upon stimulation with gingipains from Porphyromonas gingivalis (2008), J. Med. Microbiol., 57, 500-507.
    View publication on PubMed
Search for more literature for 3.4.22.37

Activating Compound

Activating Compound Comment Organism Structure
cysteine gingipains are activated by diluting to 10 mM in 0.2 M HEPES, pH 8.0, 5 mM CaCl2 and 10 mM cysteine, and incubation at 37°C for 10 min Porphyromonas gingivalis

Protein Variants

Protein Variants Comment Organism
additional information construction of a strain producing Kgp proteinase without the adhesin domains in a Rgp/Kgp/adhesin-null mutant, mutant strains 33277, KDP133, and KDP137 phenotypes, overview Porphyromonas gingivalis

Inhibitors

Inhibitors Comment Organism Structure
Phe-Pro-Arg-chloromethyl ketone i.e. FPR-cmk, a specific inhibitor of Rgps, the inhibitor almost completely negates the RgpB-induced upregulation and HRgpA-induced downregulation Porphyromonas gingivalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 x * 95000, HRgpA, SDS-PAGE, 50000, RgpB, SDS-PAGE Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Porphyromonas gingivalis HRgpA-mediated downregulation and RgpB-mediated upregulation of production of interleukin-8, occurs through protease-activated receptors, PAR-1 and PAR-2, signalling, RgpB-mediated upregulation of IL-8 production occurs through nuclear factor-kappa B, which does not affect HRgpA-mediated downregulation, overview. Gingipains preferentially suppress IL-8, resulting in attenuation of the cellular recognition of bacteria, and as a consequence, sustain chronic inflammation ?
-
 ?
additional information Porphyromonas gingivalis HG66 HRgpA-mediated downregulation and RgpB-mediated upregulation of production of interleukin-8, occurs through protease-activated receptors, PAR-1 and PAR-2, signalling, RgpB-mediated upregulation of IL-8 production occurs through nuclear factor-kappa B, which does not affect HRgpA-mediated downregulation, overview. Gingipains preferentially suppress IL-8, resulting in attenuation of the cellular recognition of bacteria, and as a consequence, sustain chronic inflammation ?
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
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 wild-type strain HG66
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Purification (Commentary)

Purification (Comment) Organism
native HRgpA and RgpB from culture supernatant Porphyromonas gingivalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information HRgpA-mediated downregulation and RgpB-mediated upregulation of production of interleukin-8, occurs through protease-activated receptors, PAR-1 and PAR-2, signalling, RgpB-mediated upregulation of IL-8 production occurs through nuclear factor-kappa B, which does not affect HRgpA-mediated downregulation, overview. Gingipains preferentially suppress IL-8, resulting in attenuation of the cellular recognition of bacteria, and as a consequence, sustain chronic inflammation Porphyromonas gingivalis ?
-
 ?
additional information HRgpA-mediated downregulation and RgpB-mediated upregulation of production of interleukin-8, occurs through protease-activated receptors, PAR-1 and PAR-2, signalling, RgpB-mediated upregulation of IL-8 production occurs through nuclear factor-kappa B, which does not affect HRgpA-mediated downregulation, overview. Gingipains preferentially suppress IL-8, resulting in attenuation of the cellular recognition of bacteria, and as a consequence, sustain chronic inflammation Porphyromonas gingivalis HG66 ?
-
 ?

Subunits

Subunits Comment Organism
More x * 95000, HRgpA, SDS-PAGE, 50000, RgpB, SDS-PAGE Porphyromonas gingivalis

Synonyms

Synonyms Comment Organism
Arginine-specific gingipain
-
 Porphyromonas gingivalis
arginine-specific gingipain 2
-
 Porphyromonas gingivalis
high-molecular-mass arginine-specific gingipain
-
 Porphyromonas gingivalis
HRgpA
-
 Porphyromonas gingivalis
RGP
-
 Porphyromonas gingivalis
RgpB
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Porphyromonas gingivalis