Protein Variants | Comment | Organism |
---|---|---|
additional information | mutant RgpA-deficient isogenic strain W501 and RgpB-deficient isogenic strain D7 show 50% of wild-type strain W50 enzyme activity | Porphyromonas gingivalis |
General Stability | Organism |
---|---|
expression of RgpB is required for correct glycosylation and stability of monomeric RgpA from strain W50 | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | - |
genes rgpA and rgpB, wild-type strain W50, RgpA-deficient isogenic strain W501, and RgpB-deficient isogenic strain D7 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | monosaccharide composition of RgpA, expression of RgpB is required for correct glycosylation and stability of monomeric RgpA from strain W50 | Porphyromonas gingivalis |
Purification (Comment) | Organism |
---|---|
native enzymes by ammonium sulfate fractionation, ion exchange and affinity chromatography, RgpA and RgpB by different methods, overview | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Nalpha-benzoyl-D,L-Arg-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | Nalpha-benzoyl-D,L-Arg + 4-nitroaniline | - |
? | |
Nalpha-benzoyl-L-Arg-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | Nalpha-benzoyl-L-Arg + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | heterodimeric RgpA fused to adhesin, i.e. HRgpA | Porphyromonas gingivalis |
monomer | RgpA | Porphyromonas gingivalis |
Synonyms | Comment | Organism |
---|---|---|
Arg-gingipain | - |
Porphyromonas gingivalis |
RgpA | - |
Porphyromonas gingivalis |
RgpB | - |
Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.1 | - |
assay at | Porphyromonas gingivalis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.3 | stability of RgpA catalytic domain of wild-type and D7 mutant strain at 30°C in absence or presence of 2-mercaptoethanol or Ca2+, overview | Porphyromonas gingivalis |