Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | substrate binding increases the dimerization affinity and activity of caspase-1 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) Star cells as insoluble inclusion bodies | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C285A | active site null mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone | irreversible active site inhibitor | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-interleukin-1beta + H2O | Homo sapiens | - |
interleukin-1beta propeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P29466 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni-NTA column chromatography and Superdex 200 gel filtration | Homo sapiens |
Renatured (Comment) | Organism |
---|---|
50 mM HEPES (pH 8.0), 100 mM NaCl, 10% (w/v) sucrose, 1 M nondetergent sulfobetaine 201, and 10 mM dithiothreitol | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-WEHD-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | N-acetyl-WEHD + 7-amino-4-trifluoromethylcoumarin | - |
? | |
pro-interleukin-1beta + H2O | - |
Homo sapiens | interleukin-1beta propeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer or dimer | mature caspase-1 exists predominantly as a monomer under physiological concentration that undergoes dimerization in the presence of substrate | Homo sapiens |