Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 Codon Plus cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme mutants in complex with 3-[2-(2-benzyloxycarbonylamino-3-methyl-butyrylamino)-propionylamino]-4-oxo-pentanoic acid, hanging drop vapor diffusion method, using 0.1 M PIPES pH 6.0, 75-175 mM (NH4)2SO4, 25% (w/v) PEG 2000 MME, 10 mM dithiothreitol, 3 mM NaN3, and 2 mM MgCl2 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A329T | the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro | Homo sapiens |
C285A | inactive | Homo sapiens |
K319R | the mutant demonstrates decreased enzymatic and interleukin-1beta releasing activity in vitro | Homo sapiens |
L265S | the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro | Homo sapiens |
N263S | the mutant demonstrates decreased enzymatic and interleukin-1beta releasing activity in vitro | Homo sapiens |
R221C | the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro | Homo sapiens |
R240Q | the mutant demonstrates decreased enzymatic and interleukin-1beta releasing activity in vitro | Homo sapiens |
T267I | the mutant demonstrates absent enzymatic and interleukin-1beta releasing activity in vitro | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10244 | - |
1 * 19844 + 1 * 10244, calculated from amino acid sequence | Homo sapiens |
19844 | - |
1 * 19844 + 1 * 10244, calculated from amino acid sequence | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pro-interleukin-1beta + H2O | Homo sapiens | - |
interleukin-1beta propeptide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P29466 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
THP-1 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-WEHD-7-amido-4-trifluoromethylcoumarin + H2O | - |
Homo sapiens | N-acetyl-WEHD + 7-amino-4-trifluoromethylcoumarin | - |
? | |
pro-interleukin-1beta + H2O | - |
Homo sapiens | interleukin-1beta propeptide | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 19844 + 1 * 10244, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Casp1 | - |
Homo sapiens |
ICE | - |
Homo sapiens |
interleukin-1 converting enzyme | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | decreased enzymatic activity of caspase-1 is compatible with normal life and does not prevent moderate and severe autoinflammation | Homo sapiens |
physiological function | caspase-1 is a proinflammatory enzyme that plays pivotal roles in innate immunity and many inflammatory conditions such as periodic fever syndromes and gout | Homo sapiens |