Cloned (Comment) | Organism |
---|---|
recombinant expression of the His-tagged enzyme in Escherichia coli inclusion bodies | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
chymostatin | - |
Entamoeba histolytica | |
E-64 | - |
Entamoeba histolytica | |
Nalpha-4-tosyl-L-lysine chloromethylketone | - |
Entamoeba histolytica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35500 | - |
x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE | Entamoeba histolytica |
52000 | - |
x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE | Entamoeba histolytica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Entamoeba histolytica | the cysteine proteinase EhCP112 and the adhesin EhADH112 assemble to form the EhCPADH complex involved in Entamoeba histolytica virulence | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the recombinant enzyme needs to be refolded and processed from the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112. The thiol specific inhibitor E-64, but not serine or aspartic proteinase inhibitors arrests this activation process | Entamoeba histolytica |
Purification (Comment) | Organism |
---|---|
recombinant refolded His-tagged enzyme by nickel affinity chromatography | Entamoeba histolytica |
Renatured (Comment) | Organism |
---|---|
recombinant His-tagged enzyme, solvation in 20 mM sodium phosphate, pH 7.4, 0.5 M NaCl, 10 mM imidazole, and 8 M urea, refolding and processing of the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112 | Entamoeba histolytica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1450 | - |
refolded, recombinant, activated enzyme | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
azocasein + H2O | - |
Entamoeba histolytica | ? | - |
? | |
additional information | the cysteine proteinase EhCP112 and the adhesin EhADH112 assemble to form the EhCPADH complex involved in Entamoeba histolytica virulence | Entamoeba histolytica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE | Entamoeba histolytica |
Synonyms | Comment | Organism |
---|---|---|
EhCP112 | - |
Entamoeba histolytica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at, substrate azocasein | Entamoeba histolytica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 60 | - |
Entamoeba histolytica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at, substrate azocasein | Entamoeba histolytica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Entamoeba histolytica |