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Literature summary for 3.4.22.35 extracted from

  • Quintas-Granados, L.I.; Orozco, E.; Brieba, L.G.; Arroyo, R.; Ortega-Lopez, J.
    Purification, refolding and autoactivation of the recombinant cysteine proteinase EhCP112 from Entamoeba histolytica (2009), Protein Expr. Purif., 63, 26-32.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of the His-tagged enzyme in Escherichia coli inclusion bodies Entamoeba histolytica

Inhibitors

Inhibitors Comment Organism Structure
chymostatin
-
Entamoeba histolytica
E-64
-
Entamoeba histolytica
Nalpha-4-tosyl-L-lysine chloromethylketone
-
Entamoeba histolytica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35500
-
x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE Entamoeba histolytica
52000
-
x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE Entamoeba histolytica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Entamoeba histolytica the cysteine proteinase EhCP112 and the adhesin EhADH112 assemble to form the EhCPADH complex involved in Entamoeba histolytica virulence ?
-
?

Organism

Organism UniProt Comment Textmining
Entamoeba histolytica
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification the recombinant enzyme needs to be refolded and processed from the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112. The thiol specific inhibitor E-64, but not serine or aspartic proteinase inhibitors arrests this activation process Entamoeba histolytica

Purification (Commentary)

Purification (Comment) Organism
recombinant refolded His-tagged enzyme by nickel affinity chromatography Entamoeba histolytica

Renatured (Commentary)

Renatured (Comment) Organism
recombinant His-tagged enzyme, solvation in 20 mM sodium phosphate, pH 7.4, 0.5 M NaCl, 10 mM imidazole, and 8 M urea, refolding and processing of the inactive precursor, ppEhCP112, to a 35.5 kDa mature and active enzyme, EhCP112 Entamoeba histolytica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1450
-
refolded, recombinant, activated enzyme Entamoeba histolytica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
azocasein + H2O
-
Entamoeba histolytica ?
-
?
additional information the cysteine proteinase EhCP112 and the adhesin EhADH112 assemble to form the EhCPADH complex involved in Entamoeba histolytica virulence Entamoeba histolytica ?
-
?

Subunits

Subunits Comment Organism
? x * 52000, recombinant and refolded enzyme, SDS-PAGE, x * 35500, recombinant, refolded, and processed enzyme, SDS-PAGE Entamoeba histolytica

Synonyms

Synonyms Comment Organism
EhCP112
-
Entamoeba histolytica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at, substrate azocasein Entamoeba histolytica

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5 60
-
Entamoeba histolytica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at, substrate azocasein Entamoeba histolytica

pH Range

pH Minimum pH Maximum Comment Organism
3 8
-
Entamoeba histolytica