Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli BL21(DE3), formation of inclusion bodies | Entamoeba histolytica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EhICP1 | Entamoeba histolytica inhibitor for cysteine protease type 1; Entamoeba histolytica inhibitor for cysteine protease type 1; Entamoeba histolytica inhibitor for cysteine protease type 1, inhibition also by regulation of CP5 processing | Entamoeba histolytica | |
EhICP2 | Entamoeba histolytica inhibitor for cysteine protease type 2; Entamoeba histolytica inhibitor for cysteine protease type 2; Entamoeba histolytica inhibitor for cysteine protease type 2, inhibition also by regulation of CP5 processing | Entamoeba histolytica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Entamoeba histolytica | 5764 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Entamoeba histolytica | essential for pathogenicity of Entamoeba histolytica | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Entamoeba histolytica | - |
- |
- |
Entamoeba histolytica | Q01957 | - |
- |
Entamoeba histolytica | Q01958 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag under denaturating conditions | Entamoeba histolytica |
Renatured (Comment) | Organism |
---|---|
dialysis against refolding buffer | Entamoeba histolytica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O | - |
Entamoeba histolytica | ? | - |
? | |
additional information | essential for pathogenicity of Entamoeba histolytica | Entamoeba histolytica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CP1 | - |
Entamoeba histolytica |
CP2 | - |
Entamoeba histolytica |
CP5 | - |
Entamoeba histolytica |
cysteine protease 1 | - |
Entamoeba histolytica |
cysteine protease 2 | - |
Entamoeba histolytica |
cysteine protease 5 | - |
Entamoeba histolytica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000005 | - |
EhICP2 | pH 5.0, 37°C | Entamoeba histolytica | |
0.00000013 | - |
EhICP1 | pH 5.0, 37°C | Entamoeba histolytica | |
0.00000017 | - |
EhICP2 | pH 5.0, 37°C | Entamoeba histolytica | |
0.00000059 | - |
EhICP1 | pH 5.0, 37°C | Entamoeba histolytica | |
0.00000081 | - |
EhICP1 | pH 5.0, 37°C | Entamoeba histolytica | |
0.00000095 | - |
EhICP2 | pH 5.0, 37°C | Entamoeba histolytica |