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Literature summary for 3.4.22.35 extracted from
- Affinity purification and biochemical characterization of histolysin, the major cysteine proteinase of Entamoeba histolytica (1988), Biochem. J., 250, 903-909.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| chicken cystatin | - |
Entamoeba histolytica | |
| iodoacetic acid | - |
Entamoeba histolytica |  |
| L-trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane | - |
Entamoeba histolytica | |
| Peptidyldiazomethanes | - |
Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0015 | - |
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide | - |
Entamoeba histolytica | |
| 0.032 | - |
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide | - |
Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 26000 | - |
1 * 26000, Entamoeba histolytica, SDS-PAGE | Entamoeba histolytica |
| 29000 | - |
Entamoeba histolytica, gel filtration | Entamoeba histolytica |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Entamoeba histolytica | role in tissue invasion | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Entamoeba histolytica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azocasein + H2O | - |
Entamoeba histolytica | fragments of azocasein | - |
? | |
| Azocoll + H2O | - |
Entamoeba histolytica | Hydrolyzed azocoll | - |
? | |
| Benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| Benzyloxycarbonyl-Phe-L-citrullin 4-methylcoumarin 7-amide + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| Cartilage proteoglycan + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| hide powder azure + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| Kidney glomerular basement-membrane collagen + H2O | - |
Entamoeba histolytica | ? | - |
? | |
| additional information | elastin | Entamoeba histolytica | ? | - |
? | |
| additional information | not: type I collagen | Entamoeba histolytica | ? | - |
? | |
| additional information | role in tissue invasion | Entamoeba histolytica | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 26000, Entamoeba histolytica, SDS-PAGE | Entamoeba histolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Entamoeba histolytica |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | - |
pH 5.5-9.5, stable during overnight incubation | Entamoeba histolytica |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
Benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide | - |
Entamoeba histolytica | |
| 130 | - |
benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide | - |
Entamoeba histolytica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
azocasein | Entamoeba histolytica |
| 6.7 | - |
hide powder azure | Entamoeba histolytica |
| 7.5 | - |
benzyloxycarbonyl-Phe-L-citrullin 4-methylcoumarin 7-amide, azocoll | Entamoeba histolytica |
| 9.5 | - |
benzyloxycarbonyl-Phe-Arg 4-methylcoumarin 7-amide, benzyloxycarbonyl-Arg-Arg 4-methylcoumarin 7-amide | Entamoeba histolytica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | 4°C, stable during overnight incubation | Entamoeba histolytica |
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