Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
lysosome | - |
Homo sapiens | 5764 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q99538 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyloxycarbonyl-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | the canonical Asn P1 specificity is only observed at pH 6.0, whereas a decrease in pH to 5.0 or even 4.0 results in a substantially increased number of cleavages of which almost half are after Asp | Homo sapiens | benzyloxycarbonyl-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? | |
benzyloxycarbonyl-Ala-Ala-Asp-7-amido-4-methylcoumarin + H2O | the canonical Asn P1 specificity is only observed at pH 6.0, whereas a decrease in pH to 5.0 or even 4.0 results in a substantially increased number of cleavages of which almost half are after Asp | Homo sapiens | benzyloxycarbonyl-Ala-Ala-Asp + 7-amino-4-methylcoumarin | - |
? | |
additional information | a gel based label-free proteomic approach (DIPPS-direct in-gel profiling of protease specificity) is developed that enables quick and reliable determination of protease cleavage specificities under large variety of experimental conditions. The methodology is based on in-gel digestion of the gel-separated proteome with the studied protease, enrichment of cleaved peptides by gel extraction, and subsequent mass spectrometry analysis combined with a length-limited unspecific database search. Specificity profiling of legumain at pH 4.0 reveales a pH-dependent change in the specificity of this protease. The canonical Asn P1 specificity is only observed at pH 6.0, whereas a decrease in pH to 5.0 or even 4.0 results in a substantially increased number of cleavages of which almost half are after Asp | Homo sapiens | ? | - |
? |
General Information | Comment | Organism |
---|---|---|
physiological function | potential role of legumain in differential substrate recognition during endolysosomal maturation | Homo sapiens |