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Literature summary for 3.4.22.34 extracted from

  • Santos-Silva, L.K.; Soares-Costa, A.; Gerald, L.T.; Meneghin, S.P.; Henrique-Silva, F.
    Recombinant expression and biochemical characterization of sugarcane legumain (2012), Plant Physiol. Biochem., 57, 181-192.
    View publication on PubMed
Search for more literature for 3.4.22.34

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Saccharum sp.

Inhibitors

Inhibitors Comment Organism Structure
cystatin 3 strong inhibition Saccharum sp.
iodoacetamide irreversible millimolar inhibitor Saccharum sp.
additional information E-64 has no effect on enzyme activity Saccharum sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, SDS-PAGE Saccharum sp.

Organism

Organism UniProt Comment Textmining
Saccharum sp. H2EUL5 sugarcane cultivar SP80-3280
-

Source Tissue

Source Tissue Comment Organism Textmining
apical meristem
-
 Saccharum sp.
-
internode
-
 Saccharum sp.
-
leaf
-
 Saccharum sp.
-
leaf roll
-
 Saccharum sp.
-
plant bud germinating, lateral Saccharum sp.
-
root band
-
 Saccharum sp.
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Saccharum sp. Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?
Z-Val-Ala-Asn-7-amido-4-methylcoumarin + H2O
-
 Saccharum sp. Z-Val-Ala-Asn + 7-amino-4-methylcoumarin
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Saccharum sp.

Synonyms

Synonyms Comment Organism
LeG
-
 Saccharum sp.
vacuolar processing enzyme
-
 Saccharum sp.
VPE
-
 Saccharum sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Saccharum sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30
-
 high activity (90%) is observed at 30°C, while a gradual loss of activity is observed after the prolonged exposure to higher temperatures Saccharum sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4 10 the enzyme is less affected when incubated at 4 or 10°C for 1 h Saccharum sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Saccharum sp.

pH Range

pH Minimum pH Maximum Comment Organism
4 8 no activity below pH 4.0 or above pH 8.0 Saccharum sp.

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.28
-
 cystatin 3 pH and temperature not specified in the publication Saccharum sp.

Expression

Organism Comment Expression
Saccharum sp. the enzyme expression is down-regulated in plantlets treated with salicylic acid down
Saccharum sp. enzyme expression is not affected by jasmonic acid and gibberellic acid additional information
Saccharum sp. the enzyme expression is up-regulated in plantlets treated with abscisic acid up