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Literature summary for 3.4.22.34 extracted from
- Phylogenetically distant barley legumains have a role in both seed and vegetative tissues (2013), J. Exp. Bot., 64, 2929-2941.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris KM71H cells | Hordeum vulgare |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cystatin CPI-4 | - |
Hordeum vulgare |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum | - |
Hordeum vulgare | 5783 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
x * 41000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare | - |
cultivar Bomi | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation | Hordeum vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aleurone | - |
Hordeum vulgare | - |
| embryo | - |
Hordeum vulgare | - |
| endosperm | - |
Hordeum vulgare | - |
| leaf | - |
Hordeum vulgare | - |
| seed | - |
Hordeum vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Hordeum vulgare | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41000, recombinant enzyme, SDS-PAGE | Hordeum vulgare |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Leg-2 | isoform | Hordeum vulgare |
| Leg-4 | isoform | Hordeum vulgare |
| vacuolar processing enzyme | - |
Hordeum vulgare |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Hordeum vulgare | isoform Leg-4 is repressed after abscisic acid exposure | down |
| Hordeum vulgare | isoform Leg-2 mRNA expression responds in leaves to biotic and abiotic stimuli, such as salicylic acid, jasmonic acid, nitric oxide, abscisic acid, and aphid infestation, and is induced by gibberellic acid in seeds. Isoform Leg-4 responds in leaves to wounding, nitric oxide, and abscisic acid treatments | up |
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Release 2023.1 (January 2023)
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