Literature summary for 3.4.22.34 extracted from

Oliver, E.M.; Skuce, P.J.; McNair, C.M.; Knox, D.P.
Identification and characterization of an asparaginyl proteinase (legumain) from the parasitic nematode, Haemonchus contortus (2006), Parasitology, 133, 237-244.

Search for more literature for 3.4.22.34

Cloned(Commentary)

Cloned (Comment)	Organism
gene leg-1, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, expression in Escherichia coli as GST-fusion protein	Haemonchus contortus

Inhibitors

Inhibitors	Comment	Organism	Structure
1,10-phenanthroline	27.5% inhibition at 5 mM	Haemonchus contortus
4-(2-aminoethyl) benzenesulfonyl fluoride	AEBSF, 45.8% inhibition at 5 mM	Haemonchus contortus
E64	17.1% inhibition at 0.001 mM	Haemonchus contortus
leupeptin	47.3% inhibition at 1 mM	Haemonchus contortus
N-ethylmaleimide	complete inhibition at 2 mM	Haemonchus contortus
pepstatin A	8.4% inhibition at 0.1 mM	Haemonchus contortus
Z-Phe-Ala-CHN2	i.e. benzyloxycarbonyl-phenylalanyl-alanyl-diazomethane, 40.8% inhibition at 0.01 mM	Haemonchus contortus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	bound	Haemonchus contortus	16020	-
microvillus	surface, of intestinal cells	Haemonchus contortus	5902	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
49000	-	x * 49000, about, amino acid sequence calculation, x * 75000, recombinant GST-fusion enzyme, SDS-PAGE	Haemonchus contortus
75000	-	x * 49000, about, amino acid sequence calculation, x * 75000, recombinant GST-fusion enzyme, SDS-PAGE	Haemonchus contortus

Organism

Organism	UniProt	Comment	Textmining
Haemonchus contortus	Q2UVF3	gene leg-1; barber pole worm, parasitic nematode	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
intestine	microvillar surface	Haemonchus contortus	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.35	-	membrane-bound fraction	Haemonchus contortus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O	-	Haemonchus contortus	Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
?	x * 49000, about, amino acid sequence calculation, x * 75000, recombinant GST-fusion enzyme, SDS-PAGE	Haemonchus contortus

Synonyms

Synonyms	Comment	Organism
asparaginyl proteinase	-	Haemonchus contortus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Haemonchus contortus

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Release 2023.1 (January 2023)