Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | - |
Phaseolus vulgaris | |
DTT | - |
Glycine max | |
DTT | - |
Arabidopsis thaliana | |
DTT | - |
Ricinus communis | |
DTT | - |
Canavalia ensiformis | |
DTT | - |
Vigna aconitifolia | |
DTT | - |
Vigna mungo | |
EDTA | - |
Phaseolus vulgaris | |
EDTA | - |
Glycine max | |
EDTA | - |
Arabidopsis thaliana | |
EDTA | - |
Ricinus communis | |
EDTA | - |
Canavalia ensiformis | |
EDTA | - |
Vigna aconitifolia | |
EDTA | - |
Vigna mungo |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination | Ricinus communis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | - |
Canavalia ensiformis | |
additional information | no inhibition by E64 | Canavalia ensiformis | |
N-ethylmaleimide | - |
Canavalia ensiformis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Phaseolus vulgaris | - |
- |
soluble | - |
Glycine max | - |
- |
soluble | - |
Arabidopsis thaliana | - |
- |
soluble | - |
Ricinus communis | - |
- |
soluble | - |
Canavalia ensiformis | - |
- |
soluble | - |
Vigna aconitifolia | - |
- |
soluble | - |
Vigna mungo | - |
- |
vacuole | - |
Phaseolus vulgaris | 5773 | - |
vacuole | - |
Glycine max | 5773 | - |
vacuole | - |
Ricinus communis | 5773 | - |
vacuole | - |
Canavalia ensiformis | 5773 | - |
vacuole | - |
Vigna aconitifolia | 5773 | - |
vacuole | - |
Vigna mungo | 5773 | - |
vacuole | the alpha and gamma vacuolar processing enzyme occurs only in vegetative organs, while the beta VPE occurs in seeds | Arabidopsis thaliana | 5773 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23400 | - |
1 * 23400 | Phaseolus vulgaris |
33000 | - |
1 * 33000 | Vigna aconitifolia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ricinus communis | the enzyme is involved in degradation of seed storage proteins | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Canavalia ensiformis | - |
jackbean | - |
Glycine max | - |
- |
- |
Phaseolus vulgaris | - |
- |
- |
Ricinus communis | - |
- |
- |
Vigna aconitifolia | - |
moth bean | - |
Vigna mungo | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | probably N-glycosylated | Vigna aconitifolia |
proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Phaseolus vulgaris |
proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Arabidopsis thaliana |
proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Ricinus communis |
proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Canavalia ensiformis |
proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Vigna aconitifolia |
proteolytic modification | autoactivation by removal of the propeptide, activation after arrival in the vacuole | Vigna mungo |
Purification (Comment) | Organism |
---|---|
native enzyme from dry seeds, to homogeneity | Glycine max |
native enzyme from dry seeds, to homogeneity | Ricinus communis |
native enzyme from dry seeds, to homogeneity | Canavalia ensiformis |
native enzyme from germinated seeds, to homogeneity | Phaseolus vulgaris |
native enzyme from germinated seeds, to homogeneity | Vigna aconitifolia |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | late stage of seed maturation | Ricinus communis | - |
additional information | the alpha and gamma vacuolar processing enzyme occurs only in vegetative organs | Arabidopsis thaliana | - |
seed | - |
Vigna aconitifolia | - |
seed | beta VPE | Arabidopsis thaliana | - |
seed | in endosperm of late stage of seed maturation | Ricinus communis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Boc-Asn-4-nitrophenyl ester + H2O | - |
Phaseolus vulgaris | Boc-Asn + 4-nitrophenol | - |
? | |
Dnp-Pro-Glu-Ala-Asn-NH2 + H2O | - |
Phaseolus vulgaris | Dnp-Pro-Glu-Ala-Asn + NH3 | - |
? | |
ETRNGVEE + H2O | - |
Phaseolus vulgaris | ETRN + GVEE | - |
? | |
ETRNGVEE + H2O | - |
Glycine max | ETRN + GVEE | - |
? | |
additional information | the enzyme is involved in degradation of seed storage proteins | Ricinus communis | ? | - |
? | |
additional information | the enzyme hydrolyzes peptide bonds with Asn in P1 | Phaseolus vulgaris | ? | - |
? | |
additional information | the enzyme hydrolyzes peptide bonds with Asn in P1 and all 20 amino acids in P1' including S-alkylated Cys, with a few exceptions | Canavalia ensiformis | ? | - |
? | |
SESENGLEET + H2O | - |
Phaseolus vulgaris | SESEN + GLEET | - |
? | |
SESENGLEET + H2O | - |
Glycine max | SESEN + GLEET | - |
? | |
Z-Ala-Ala-Asn-7-amido-4-methylcoumarin + H2O | - |
Phaseolus vulgaris | Z-Ala-Ala-Asn + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000 | Vigna aconitifolia |
monomer | 1 * 23400 | Phaseolus vulgaris |
monomer | 1 * 33000-33800 | Glycine max |
More | primary structure determination | Ricinus communis |
Synonyms | Comment | Organism |
---|---|---|
Asparaginyl endopeptidase | - |
Phaseolus vulgaris |
Asparaginyl endopeptidase | - |
Glycine max |
Asparaginyl endopeptidase | - |
Arabidopsis thaliana |
Asparaginyl endopeptidase | - |
Ricinus communis |
Asparaginyl endopeptidase | - |
Canavalia ensiformis |
Asparaginyl endopeptidase | - |
Vigna aconitifolia |
Asparaginyl endopeptidase | - |
Vigna mungo |
vacuolar processing enzyme | - |
Phaseolus vulgaris |
vacuolar processing enzyme | - |
Glycine max |
vacuolar processing enzyme | - |
Arabidopsis thaliana |
vacuolar processing enzyme | - |
Ricinus communis |
vacuolar processing enzyme | - |
Canavalia ensiformis |
vacuolar processing enzyme | - |
Vigna aconitifolia |
vacuolar processing enzyme | - |
Vigna mungo |
VPE | - |
Phaseolus vulgaris |
VPE | - |
Glycine max |
VPE | - |
Arabidopsis thaliana |
VPE | - |
Ricinus communis |
VPE | - |
Canavalia ensiformis |
VPE | - |
Vigna aconitifolia |
VPE | - |
Vigna mungo |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 5.5 | - |
Phaseolus vulgaris |
5 | 5.5 | - |
Glycine max |
5 | 5.5 | - |
Arabidopsis thaliana |
5 | 5.5 | - |
Ricinus communis |
5 | 5.5 | - |
Canavalia ensiformis |
5 | 5.5 | - |
Vigna aconitifolia |
5 | 5.5 | - |
Vigna mungo |